{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T13:14:40Z","timestamp":1781788480924,"version":"3.54.5"},"reference-count":41,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2016,1,29]],"date-time":"2016-01-29T00:00:00Z","timestamp":1454025600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001407","name":"Department of Biotechnology , Ministry of Science and Technology","doi-asserted-by":"publisher","award":["BT\/06\/IYBA\/2012."],"award-info":[{"award-number":["BT\/06\/IYBA\/2012."]}],"id":[{"id":"10.13039\/501100001407","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001407","name":"Department of Biotechnology , Ministry of Science and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001407","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Biomed Mater Res"],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In this study, platelet\u2010rich plasma (PRP) was incorporated into gelatin\u2010nanohydroxyapatite fibrous scaffold in two forms (PRP gel as coating on the scaffold [PCSC] and PRP powder within the scaffold [PCSL] and investigated for (a) growth factor release; (b) stability of scaffold at different temperature; (c) stability of scaffold before and after ETO sterilization; and (d) osteogenic and endothelial differentiation potential using mesenchymal stem cells (MSCs). PCSC demonstrated a high and burst growth factor release initially followed by a gradual reduction in its concentration, while PCSL showed a steady state release pattern for 30 days. The stability of growth factors released from PCSL was not altered either through ETO sterilization or through its storage at different temperature. PRP\u2010loaded scaffolds induced the differentiation of MSCs into osteogenic and endothelial lineage without providing any induction factors in the cell culture medium and the differentiation rate was significantly higher when compared to the scaffolds devoid of PRP. PCSC performed better than PCSL. In general, PRP in combination with composite fibrous scaffold could be a promising candidate for bone tissue engineering applications. \u00a9 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 104B: 771\u2013781, 2016.<\/jats:p>","DOI":"10.1002\/jbm.b.33605","type":"journal-article","created":{"date-parts":[[2016,1,29]],"date-time":"2016-01-29T06:03:22Z","timestamp":1454047402000},"page":"771-781","source":"Crossref","is-referenced-by-count":16,"title":["Evaluation of osteoinductive and endothelial differentiation potential of Platelet\u2010Rich Plasma incorporated Gelatin\u2010Nanohydroxyapatite Fibrous Matrix"],"prefix":"10.1002","volume":"104","author":[{"given":"Anjana","family":"J","sequence":"first","affiliation":[{"name":"Amrita Center for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences &amp; Research Center, Amrita Vishwa Vidyapeetham University Kochi 682041 Kerala India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shruthy","family":"Kuttappan","sequence":"additional","affiliation":[{"name":"Amrita Center for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences &amp; Research Center, Amrita Vishwa Vidyapeetham University Kochi 682041 Kerala India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kripa S.","family":"Keyan","sequence":"additional","affiliation":[{"name":"Amrita Center for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences &amp; Research Center, Amrita Vishwa Vidyapeetham University Kochi 682041 Kerala India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manitha B.","family":"Nair","sequence":"additional","affiliation":[{"name":"Amrita Center for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences &amp; Research Center, Amrita Vishwa Vidyapeetham University Kochi 682041 Kerala India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,1,29]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10856-014-5240-2"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/mabi.200400026"},{"key":"e_1_2_7_4_1","first-page":"15","article-title":"Orthopaedic applications of bone graft & graft substitutes: a review","volume":"132","author":"Nandi SK","year":"2010","journal-title":"Indian J Med Res"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2015.02.006"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1622.2001.02128.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2015.01.017"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1163\/156856200743508"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0131188"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.teb.2009.0085"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.2174\/1381612811319190008"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1007502828372"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joms.2003.12.003"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0278-2391(94)90401-4"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0278-2391(97)90187-7"},{"key":"e_1_2_7_16_1","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1051\/ject\/200638174","article-title":"Platelet\u2010rich plasma and platelet gel: a review","volume":"38","author":"Everts PA","year":"2006","journal-title":"J Extra Corpor Technol"},{"key":"e_1_2_7_17_1","first-page":"392","article-title":"Platelet\u2010rich plasma in bone regeneration: engineering the delivery for improved clinical efficacy","author":"Rodriguez IA","year":"2014","journal-title":"Biomed Res Int"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1524-475X.2008.00362.x"},{"key":"e_1_2_7_19_1","first-page":"2723","article-title":"Incorporating platelet\u2010rich plasma into electrospun scaffolds for tissue engineering applications. Tissue Eng","volume":"17","author":"Sell SA","year":"2011","journal-title":"Part A"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.31740"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jvs.2009.06.016"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1088\/0957-4484\/26\/40\/405101"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijom.2004.10.018"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1080\/08977190802636713"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/1479-5876-10-243"},{"key":"e_1_2_7_26_1","first-page":"615","article-title":"Preanalytical and analytical considerations for measuring nitric oxide metabolites in serum or plasma using the Griess method","volume":"58","author":"Ghasemi A","year":"2012","journal-title":"Clin Lab"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2005.07.003"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/7.8.771"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.2215\/CJN.04151206"},{"key":"e_1_2_7_30_1","first-page":"1","article-title":"Calcium phosphate nanocoatings and nanocomposites, Part I: recent developments and advancements in tissue engineering and bioimaging","volume":"29","author":"Choi AH","year":"2015","journal-title":"Nanomedicine (Lond)"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2006.01.002"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.34248"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2010.0004"},{"key":"e_1_2_7_34_1","first-page":"1","article-title":"Controlled release of vascular endothelial growth factor from spray\u2010dried alginate microparticles in collagen\u2010hydroxyapatite scaffolds for promoting vascularization and bone repair","volume":"10","author":"Quinlan E","year":"2015","journal-title":"J Tissue Eng Regen Med"},{"key":"e_1_2_7_35_1","first-page":"207","article-title":"Development of a cost\u2010effective method for platelet\u2010rich plasma (PRP) preparation for topical wound healing","volume":"25","author":"Akhundov K","year":"2012","journal-title":"Ann Burns Fire Disasters"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/176060"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.b.30886"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.athoracsur.2011.04.084"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/396131"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.14.7192"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00264-003-0524-z"},{"key":"e_1_2_7_42_1","first-page":"1380","article-title":"Biological stability of plasma rich in growth factors (PRGF\u2010Endoret) eye drops at different temperature conditions for 24 hours","volume":"55","author":"Orive G","year":"2014","journal-title":"Invest Ophthalmol Vis Sci"}],"container-title":["Journal of Biomedical Materials Research Part B: Applied Biomaterials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjbm.b.33605","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbm.b.33605","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,12]],"date-time":"2023-09-12T21:35:41Z","timestamp":1694554541000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbm.b.33605"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,1,29]]},"references-count":41,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2016,5]]}},"alternative-id":["10.1002\/jbm.b.33605"],"URL":"https:\/\/doi.org\/10.1002\/jbm.b.33605","archive":["Portico"],"relation":{},"ISSN":["1552-4973","1552-4981"],"issn-type":[{"value":"1552-4973","type":"print"},{"value":"1552-4981","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,1,29]]}}}