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The development of a tissue\u2010engineered left ventricle (LV) would provide a therapeutic option to overcome the inefficiencies and limitations associated with current treatment options. This study provides a foundation for the development and fabrication of the bioengineered open ventricle (BEOV) model. BEOV molds were developed to emulate the human LV geometry; molds were used to produce chitosan scaffolds. BEOV were fabricated by culturing 30 million rat neonatal cardiac cells on the chitosan scaffold. The model demonstrated 57% cell retention following 4days culture. The average biopotential output for the model was 1615 \u00b5V. Histological assessment displayed the presence of localized cell clusters, with intercellular and cell\u2010scaffold interactions. The BEOV provides a novel foundation for the development of a 3D bioengineered LV for application in HLHS. \u00a9 2016 Wiley Periodicals, Inc. 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