{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T18:40:58Z","timestamp":1759776058460},"reference-count":58,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2016,11,27]],"date-time":"2016-11-27T00:00:00Z","timestamp":1480204800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31470940","31370987","81371696"],"award-info":[{"award-number":["31470940","31370987","81371696"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Biomed Mater Res"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Although iron oxide nanoparticles (IRONs) were applied in clinical magnetic resonance imaging<jats:italic>in vivo<\/jats:italic>and magnetic tissue engineering<jats:italic>in vitro<\/jats:italic>widely, the underlying effects of IRONs on the development of cardiomyocytes especially the intercellular junctions, intercalated discs (IDs), remain an unknown issue. Given the critical role of three\u2010dimensional (3D) engineered cardiac tissues (ECTs) in evaluation of nanoparticles toxicology, it remained necessary to understand the effects of IRONs on IDs assembly of cardiomyocytes in 3D environment. In this study, we first reconstituted collagen\/Matrigel based ECTs<jats:italic>in vitro<\/jats:italic>and prepared IRONs with 2,3\u2010dimercaptosuccinic acid (DMSA\u2010IRONs). We found that the internalization of DMSA\u2010IRONs by cardiac cells in dose\u2010dependent manner was not associated with the cell distribution in 3D environment by determination of Prussian blue staining and transmission electronic microscopy. Significantly, through determination of western blotting and immunofluorescence of connexin 43, N\u2010cadherin, desmoplakin, and plakoglobin, DMSA\u2010IRONs enhanced the assembly of gap junctions, decreased mechanical junctions (adherens junctions and desmosomes) of cardiac cells but not in dose\u2010dependent manner in ECTs at seventh day. In addition, DMSA\u2010IRONs increased the vesicles secretion of cardiac cells in ECTs apparently compared to control groups. Overall, we conclude that the internalization of DMSA\u2010IRONs by cardiac cells in dose\u2010dependent manner enhanced the assembly of electrochemical junctions and decreased the mechanical related microstructures. \u00a9 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 121\u2013130, 2018.<\/jats:p>","DOI":"10.1002\/jbm.b.33757","type":"journal-article","created":{"date-parts":[[2016,11,27]],"date-time":"2016-11-27T11:12:43Z","timestamp":1480245163000},"page":"121-130","source":"Crossref","is-referenced-by-count":15,"title":["Effects of different doses of 2,3\u2010dimercaptosuccinic acid\u2010modified Fe<sub>2<\/sub>O<sub>3<\/sub>nanoparticles on intercalated discs in engineered cardiac tissues"],"prefix":"10.1002","volume":"106","author":[{"given":"Yongchao","family":"Mou","sequence":"first","affiliation":[{"name":"Department of Advanced Interdisciplinary Studies Institute of Basic Medical Sciences and Tissue Engineering Research Center, Academy of Military Medical Sciences Beijing People's Republic of China"},{"name":"School of Life Science and Technology Harbin Institute of Technology Harbin People's Republic of 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