{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T01:10:41Z","timestamp":1792113041176,"version":"4.4.0"},"reference-count":43,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,3,9]],"date-time":"2017-03-09T00:00:00Z","timestamp":1489017600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Biomed Mater Res"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Bone defects created after curettage of benign bone tumors are customarily filled with solid poly(methyl methacrylate) (PMMA) or other bone substitutes. In this study, we depicted a porous PMMA\u2010based cement (produced by mixing sodium bicarbonate and citric acid) and evaluated the prospect of its clinic application. Cement samples were characterized by high\u2010performance liquid chromatography (HPLC) coupled to mass spectrometry and its cytotoxicity evaluated in fibroblast cultures. Implantation in rabbits allowed the histologic analysis of bone, kidneys, and liver for toxicity and coagulation tests, and MRI images for hemostasis evaluation. Osseointegration was analyzed through radiography, microtomography (micro\u2010CT), SEM, and histology of sheep specimens. Rabbit specimens were analyzed 1, 4, and 7 days after implantation of porous or solid bone cement in 6.0 mm femoral defects. Sheep specimens were analyzed 3 and 6 months after implantation or not of porous or solid cement in 15.0 mm subchondral tibial defects. The production process did not release any detectable toxic substance but slightly reduced fibroblast proliferation\n                    <jats:italic>in vitro<\/jats:italic>\n                    . Until 7 days after surgery, no local or systemic alterations could be detected in histology, or hematoma formation in histology or MRI. Sheep implants showed 6 mm linear ingrowth from the bone\u2013cement interface and 20% bone ingrowth considering the whole defect area. Radiography, micro\u2010CT, SEM, and histology confirmed these findings. We conclude that our porous PMMA\u2010based cement is an attractive alternative treatment for bone defect filling that combines osseointegration and early weight bearing. \u00a9 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 649\u2013658, 2018.\n                  <\/jats:p>","DOI":"10.1002\/jbm.b.33870","type":"journal-article","created":{"date-parts":[[2017,3,9]],"date-time":"2017-03-09T00:51:47Z","timestamp":1489020707000},"page":"649-658","source":"Crossref","is-referenced-by-count":31,"title":["Safety, osseointegration, and bone ingrowth analysis of\n                    <scp>PMMA<\/scp>\n                    \u2010based porous cement on animal metaphyseal bone defect model"],"prefix":"10.1002","volume":"106","author":[{"given":"Bruno","family":"Cimatti","sequence":"first","affiliation":[{"name":"Department of Biomechanics, Medicine and Rehabilitation of the Locomotor System Ribeir\u00e3o Preto Medical School, University of S\u00e3o Paulo Ribeir\u00e3o Preto, S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana Avelino dos","family":"Santos","sequence":"additional","affiliation":[{"name":"Department of Biomechanics, Medicine and Rehabilitation of the Locomotor System Ribeir\u00e3o Preto Medical School, University of S\u00e3o Paulo Ribeir\u00e3o Preto, S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Sol","family":"Brassesco","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Philosophy, Sciences and Letters at Ribeir\u00e3o Preto University of S\u00e3o Paulo Ribeir\u00e3o Preto, S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura Tiemi","family":"Okano","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Philosophy, Sciences and Letters at Ribeir\u00e3o Preto University of S\u00e3o Paulo Ribeir\u00e3o Preto, S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wendell Monteiro","family":"Barboza","sequence":"additional","affiliation":[{"name":"Department of Biomechanics, Medicine and Rehabilitation of the Locomotor System Ribeir\u00e3o Preto Medical School, University of S\u00e3o Paulo Ribeir\u00e3o Preto, S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcello Henrique","family":"Nogueira\u2010Barbosa","sequence":"additional","affiliation":[{"name":"Division of Radiology and Diagnostic Imaging Ribeir\u00e3o Preto Medical School, University of S\u00e3o Paulo Ribeir\u00e3o Preto, S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edgard Eduard","family":"Engel","sequence":"additional","affiliation":[{"name":"Department of Biomechanics, Medicine and Rehabilitation of the Locomotor System Ribeir\u00e3o Preto Medical School, University of S\u00e3o Paulo Ribeir\u00e3o Preto, S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,3,9]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10856-014-5240-2"},{"issue":"6","key":"e_1_2_6_3_1","first-page":"576","article-title":"Porous acrylic cement: Evaluation of a novel implant material","volume":"21","author":"Mullem PJ","year":"1988","journal-title":"Ann PlastSurg"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/17453670710015571"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0736-0266(01)00124-3"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmbbm.2015.12.016"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4636(199722)38:2<155::AID-JBM10>3.0.CO;2-C"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2005.05.103"},{"issue":"6","key":"e_1_2_6_9_1","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1590\/1413-78522014220600973","article-title":"Gct: What happened after 10 years of curettage and cement\u2009? Retrospective study of 46 cases","volume":"22","author":"Baptista A","year":"2014","journal-title":"Acta Ortop Bras"},{"issue":"12","key":"e_1_2_6_10_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pone.0083662","article-title":"Microdamage caused by fatigue loading in human cancellous bone: Relationship to reductions in bone biomechanical performance","volume":"8","author":"Lambers FM","year":"2013","journal-title":"PLoS One"},{"issue":"4","key":"e_1_2_6_11_1","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1002\/jbm.820100419","article-title":"Poly(methyl methacrylate)\u2013aqueous phase blends: in situ curing porous materials","volume":"10","author":"Wijn JR.","year":"1976","journal-title":"J Biomed Mater Res"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2006.05.022"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2003.10.066"},{"issue":"1","key":"e_1_2_6_14_1","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1002\/jbm.b.31008","article-title":"The influence of the acidic component of the gas\u2010foaming porogen used in preparing an injectable porous calcium phosphate cement on its properties: acetic acid versus citric acid","volume":"86","author":"Hesaraki S","year":"2008","journal-title":"J Biomed Mater Res B Appl Biomater"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1590\/1413-785220152304145751"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2007.05.015"},{"issue":"7","key":"e_1_2_6_17_1","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1302\/0301-620X.89B7.19148","article-title":"The role of polymethylmethacrylate bone cement in modern orthopaedic surgery","volume":"89","author":"Webb JCJ","year":"2007","journal-title":"J Bone Joint Surg Br"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2007.09.011"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jbchem.a021589"},{"issue":"3","key":"e_1_2_6_20_1","doi-asserted-by":"crossref","first-page":"295","DOI":"10.4103\/0019-5413.111507","article-title":"Curettage of benign bone tumors and tumor like lesions: A retrospective analysis","volume":"47","author":"Kundu ZS","year":"2013","journal-title":"Indian J Orthop"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.BRS.0000076829.54235.9F"},{"issue":"12","key":"e_1_2_6_22_1","article-title":"Porous titanium\u20106 aluminum\u20104 vanadium cage has better osseointegration and less micromotion than a poly\u2010ether\u2010ether\u2010ketone cage in sheep vertebral fusion","volume":"37","author":"Wu SH","year":"2013","journal-title":"Artif Organs"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.20010"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1008929305897"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2006.08.013"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s001980170161"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1364\/JOSAA.1.000612"},{"issue":"4","key":"e_1_2_6_28_1","doi-asserted-by":"crossref","DOI":"10.1016\/8756-3282(95)00026-A","article-title":"The potential of sheep for the study of osteopenia: Current status and comparison with other animal models","volume":"16","author":"Newman E","year":"1995","journal-title":"Bone"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(03)00515-5"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.5312\/wjo.v4.i2.67"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jor.2016.01.002"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.b.31044"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2015.12.040"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10856-013-5030-2"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00586-013-2809-2"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4636(20000315)49:4<498::AID-JBM8>3.0.CO;2-1"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmbbm.2011.07.007"},{"issue":"9","key":"e_1_2_6_38_1","doi-asserted-by":"crossref","first-page":"3755","DOI":"10.1016\/j.actbio.2010.03.028","article-title":"Augmentation of bone defect healing using a new biocomposite scaffold: An in vivo study in sheep","volume":"6","author":"Pol U","year":"2010","journal-title":"Acta Biomater"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1080\/17453670902804505"},{"key":"e_1_2_6_40_1","doi-asserted-by":"crossref","first-page":"1\u201313","DOI":"10.1038\/srep17134","article-title":"Effect of low\u2010level mechanical vibration on osteogenesis and osseointegration of porous titanium implants in the repair of long bone defects","volume":"5","author":"Jing D","year":"2015","journal-title":"Sci Rep"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.34324"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.orthres.2005.03.021.1100230606"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2009.04.002"},{"key":"e_1_2_6_44_1","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.ddmod.2015.02.001","article-title":"Animal models in bone repair","volume":"13","author":"Rechenberg B Von.","year":"2015","journal-title":"Drug Discov Today Dis Model"}],"container-title":["Journal of Biomedical Materials Research Part B: Applied Biomaterials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjbm.b.33870","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbm.b.33870","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T02:06:57Z","timestamp":1695953217000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbm.b.33870"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,3,9]]},"references-count":43,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2018,2]]}},"alternative-id":["10.1002\/jbm.b.33870"],"URL":"https:\/\/doi.org\/10.1002\/jbm.b.33870","archive":["Portico"],"relation":{},"ISSN":["1552-4973","1552-4981"],"issn-type":[{"value":"1552-4973","type":"print"},{"value":"1552-4981","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,3,9]]}}}