{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T10:12:25Z","timestamp":1769940745101,"version":"3.49.0"},"reference-count":41,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,8,14]],"date-time":"2017-08-14T00:00:00Z","timestamp":1502668800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004484","name":"Tehran University of Medical Sciences and Health Services","doi-asserted-by":"publisher","award":["94\u201303\u2010181\u201330430"],"award-info":[{"award-number":["94\u201303\u2010181\u201330430"]}],"id":[{"id":"10.13039\/501100004484","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Biomed Mater Res"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The peripheral nerve regeneration is still one of the major clinical problems, which has received a great deal of attention. In this study, the electrospun silk fibroin (SF)\/poly(ethylene oxide) (PEO) nanofibrous scaffolds were fabricated and functionalized their surfaces with laminin (LN) without chemical linkers for potential use in the peripheral nerve tissue engineering. The morphology, surface chemistry, thermal behavior and wettability of the scaffolds were examined to evaluate their performance by means of scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, differential scanning calorimetry (DSC) and water contact angle (WCA) measurements, respectively. The proliferation and viability of Schwann cells onto the surfaces of SF\/PEO nanofibrous scaffolds were investigated using SEM and thiazolyl blue (MTT) assay. The results showed an improvement of SF conformation and surface hydrophilicity of SF\/PEO nanofibers after methanol and O<jats:sub>2<\/jats:sub> plasma treatments. The immunostaining observation indicated a continuous coating of LN on the scaffolds. Improving the surface hydrophilicity and LN functionalization significantly increased the cell proliferation and this was more prominent after 5 days of culture time. In conclusion, the obtained results revealed that the electrospun LN\u2010functionalized SF\/PEO nanofibrous scaffold could be a promising candidate for peripheral nerve tissue regeneration. \u00a9 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1595\u20131604, 2018.<\/jats:p>","DOI":"10.1002\/jbm.b.33968","type":"journal-article","created":{"date-parts":[[2017,8,14]],"date-time":"2017-08-14T06:24:42Z","timestamp":1502691882000},"page":"1595-1604","source":"Crossref","is-referenced-by-count":61,"title":["Fabrication and characterization of electrospun laminin\u2010functionalized silk fibroin\/poly(ethylene oxide) nanofibrous scaffolds for peripheral nerve regeneration"],"prefix":"10.1002","volume":"106","author":[{"given":"Mina","family":"Rajabi","sequence":"first","affiliation":[{"name":"Department of Polymer, School of Chemical Engineering, College of Engineering University of Tehran Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masoumeh","family":"Firouzi","sequence":"additional","affiliation":[{"name":"Tissue Repair Laboratory Institute of Biochemistry and Biophysics (IBB), University of Tehran Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zahra","family":"Hassannejad","sequence":"additional","affiliation":[{"name":"Pediatric Urology and Regenerative Medicine Research Center Children's Hospital Medical Center, Tehran University of Medical Sciences Tehran Iran"},{"name":"Sina Trauma and Surgery Research Center Tehran University of Medical Sciences Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ismaeil","family":"Haririan","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Biomaterials and Medical Biomaterials Research Center Faculty of Pharmacy and Department of Pharmaceutics, Tehran University of Medical Sciences, Tehran P. O. Box: 14155\u20106451 Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6636-4534","authenticated-orcid":false,"given":"Payam","family":"Zahedi","sequence":"additional","affiliation":[{"name":"Department of Polymer, School of Chemical Engineering, College of Engineering University of Tehran Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,8,14]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eurpolymj.2013.10.021"},{"issue":"3","key":"e_1_2_7_3_1","first-page":"245","article-title":"The effects of plasma treated electrospun nanofibrous poly (\u025b\u2010caprolactone) scaffolds with different orientations on mouse embryonic stem cell proliferation","volume":"16","author":"Abbasi N","year":"2014","journal-title":"Cell J"},{"issue":"3","key":"e_1_2_7_4_1","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1002\/jbm.a.33049","article-title":"Salicylic acid\u2010derived poly (anhydride\u2010ester) electrospun fibers designed for regenerating the peripheral nervous system","volume":"97","author":"Griffin J","year":"2011","journal-title":"J Biomed Mater Res A"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.b.32676"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2011.02.008"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.teb.2011.0240"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1134\/S0965545X10040097"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.polymer.2004.11.029"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/bm025581u"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/bm0345460"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M006897200"},{"issue":"170","key":"e_1_2_7_13_1","first-page":"1","article-title":"Preparation and characterization of biomimetic silk fibroin\/chitosan composite nanofibers by electrospinning for osteoblasts culture","volume":"7","author":"Chen JP","year":"2012","journal-title":"Nanoscale Res Lett"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1134\/S1070427206060012"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature01809"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/1521-3935(200207)203:10\/11<1658::AID-MACP1658>3.0.CO;2-3"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1039\/c2jm31174g"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2010.03.008"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2010.12.008"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-444-63486-3.00016-5"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0177(200006)218:2<213::AID-DVDY1>3.0.CO;2-R"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/12_2011_122"},{"issue":"1","key":"e_1_2_7_23_1","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1089\/ten.tec.2007.0366","article-title":"Laminin nanofiber meshes that mimic morphological properties and bioactivity of basement membranes","volume":"15","author":"Neal RA","year":"2008","journal-title":"Tissue Eng Part C"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1116\/1.4857295"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.33204"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0079-6123(00)28031-X"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2013.01.025"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200307068"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2008.12.008"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.3109\/10731199.2011.637927"},{"issue":"1","key":"e_1_2_7_31_1","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.memsci.2006.09.020","article-title":"Characterization of nanofibrous membranes with capillary flow porometry","volume":"286","author":"Li D","year":"2006","journal-title":"J Membr Sci"},{"issue":"1","key":"e_1_2_7_32_1","first-page":"53","article-title":"Schwann cell apoptosis and p75NTR siRNA","volume":"10","author":"Firouzi M","year":"2011","journal-title":"Iran J Allergy Asthma Immunol"},{"issue":"9","key":"e_1_2_7_33_1","first-page":"562","article-title":"Subarachnoid space transplantation of Schwann and\/or olfactory ensheathing cells following severe spinal cord injury fails to improve locomotor recovery in rats","volume":"54","author":"Nategh M","year":"2016","journal-title":"Acta Med Iran"},{"issue":"19","key":"e_1_2_7_34_1","first-page":"3138","article-title":"Identification and classification of silks using infrared spectroscopy","volume":"218","author":"Boulet\u2010Audet M","year":"2015","journal-title":"J Exp Biol"},{"key":"e_1_2_7_35_1","first-page":"134","volume-title":"Methods to Produce Silk Fibroin Film Biomaterials for Applications in Corneal Tissue Regeneration","author":"Lawrence BD.","year":"2008"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1039\/C2SM26945G"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/mabi.200500246"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2006.12.004"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2009.05.012"},{"issue":"10","key":"e_1_2_7_40_1","doi-asserted-by":"crossref","first-page":"802","DOI":"10.1007\/BF02618296","article-title":"Surface treatments and cell attachment","volume":"20","author":"Ramsey WS","year":"1984","journal-title":"In Vitro Cell Dev Biol Plant"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1469-7580.1998.19310001.x"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1088\/0957-4484\/19\/45\/455102"}],"container-title":["Journal of Biomedical Materials Research Part B: Applied Biomaterials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjbm.b.33968","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbm.b.33968","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T15:44:13Z","timestamp":1695570253000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbm.b.33968"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,8,14]]},"references-count":41,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2018,5]]}},"alternative-id":["10.1002\/jbm.b.33968"],"URL":"https:\/\/doi.org\/10.1002\/jbm.b.33968","archive":["Portico"],"relation":{},"ISSN":["1552-4973","1552-4981"],"issn-type":[{"value":"1552-4973","type":"print"},{"value":"1552-4981","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,8,14]]}}}