{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T23:29:02Z","timestamp":1789946942592,"version":"4.0.1"},"reference-count":49,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2017,9,15]],"date-time":"2017-09-15T00:00:00Z","timestamp":1505433600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"National Taipei University of Technology-Taipei Medical University Joint Research Program","award":["NTUT-TMU-104-07"],"award-info":[{"award-number":["NTUT-TMU-104-07"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Biomed Mater Res"],"published-print":{"date-parts":[[2018,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Proinflammatory cytokines and reactive oxygen species (ROS) are known to be involved in the initiation and progression of osteoarthritis (OA). New evidence clarifying the correlation between ROS and inflammation has indicated that oxidative stress can up\u2010regulate inflammatory cytokines.\n                    <jats:sc>l<\/jats:sc>\n                    \u2010Ascorbic acid (AA), an antioxidant, has been shown to have anti\u2010inflammatory effects and improve matrix deposition in chondrocytes. The purpose of this study was to examine the effects of hyaluronic acid (HA; 100 \u03bcg\/mL) supplemented with AA (50 \u03bcg\/mL) on human normal and interleukin\u20101 beta\u2010stimulated (IL\u20101\u03b2, 10 ng\/mL) chondrocytes. HA, AA, and HA\u2009+\u2009AA treatment did not change cell morphology, viability, proliferation, and glycosaminoglycan production in normal chondrocytes. HA, AA, and HA\u2009+\u2009AA, by contrast, partially restored viability and morphology of hypertrophic chondrocytes, and HA and HA\u2009+\u2009AA further decreased the cytotoxicity of IL\u20101\u03b2. Real\u2010time PCR revealed that AA and HA\u2009+\u2009AA had no substantial effects on unstimulated chondrocytes, except for down\u2010regulation of matrix metalloproteinase (MMP)\u20109 mRNA levels. For IL\u20101\u03b2\u2010stimulated chondrocytes, significant down\u2010regulation of IL\u20101\u03b2, tumor necrosis factor\u2010alpha (TNF\u2010\u03b1), MMP\u20103, and MMP\u20109 mRNA expression was found when cells were cultured in HA\u2010supplemented media. Moreover, HA\u2009+\u2009AA supplementation further significantly decreased MMP\u20103 and MMP\u20109 mRNA expression. The protein production of MMP\u20103 was decreased, with a significant difference between the HA\u2009+\u2009AA group and HA group. The antioxidant capacity and superoxide dismutases activity were also partially restored in stimulated chondrocytes. HA supplemented with AA modulates MMPs expression and antioxidant fuction in chondrocytes. AA may enhance the anticatabolic effects of HA on OA chondrocytes. \u00a9 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1809\u20131817, 2018.\n                  <\/jats:p>","DOI":"10.1002\/jbm.b.33988","type":"journal-article","created":{"date-parts":[[2017,9,15]],"date-time":"2017-09-15T10:22:49Z","timestamp":1505470969000},"page":"1809-1817","source":"Crossref","is-referenced-by-count":12,"title":["Synergistic effect of\n                    <scp>l<\/scp>\n                    \u2010ascorbic acid and hyaluronic acid on the expressions of matrix metalloproteinase\u20103 and \u22129 in human chondrocytes"],"prefix":"10.1002","volume":"106","author":[{"given":"Teng\u2010Le","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Orthopedics Tai\u2010An Hospital Shuang Shi Branch Taichung 40455 Taiwan"},{"name":"Department of Biomedical Imaging and Radiological Science, College of Medicine China Medical University Taichung 40402 Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Che\u2010Hua","family":"Yang","sequence":"additional","affiliation":[{"name":"Graduate Institute of Manufacturing Technology, National Taipei University of Technology Taipei 10608 Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Goichi","family":"Yanai","sequence":"additional","affiliation":[{"name":"Laboratory of Organ and Tissue Reconstruction Institute for Frontier Life and Medical Sciences, Kyoto University 606\u20108507 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u2010Yu","family":"Liao","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Tai\u2010An Hospital Shuang Shi Branch Taichung 40455 Taiwan"},{"name":"Department of Biomedical Imaging and Radiological Science, College of Medicine China Medical University Taichung 40402 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