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The recipient site is often prepared by laser\u2010mediated dermabrasion. Such procedures encounter disadvantages including prolonged transplantation duration, unsecured cell adherence to lesional skin and potential scarring. To improve this, here we propose a method by preparing recipient sites before transplantation by psoralen and ultraviolet A (PUVA)\u2010induced sunburn followed by transplanting cells with a chitosan\u2010based melanocyte spheroid patch. We evaluated the method in nude mice. Application of methoxsalen\u2010soaked filter paper on skin for 30 min followed by ultraviolet A exposure induced controlled sunburn blisters in 2 days. Upon transplantation, the blister roof could be quickly peeled off by a waxing patch. The chitosan membrane on which melanocytes were precultured into multicellular spheroids was transplanted with cells facing the skin. The chitosan patch adhered well to skin and secured the contact of melanocytes with the recipient site. One day later, melanocyte spheroids already detached from the chitosan membrane and adhered to the recipient skin. Our results suggest that the combination of chitosan\u2010based melanocyte spheroid patch with epidermal ablation by PUVA\u2010induced sunburn reaction can be a feasible method to facilitate melanocyte transplantation. \u00a9 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 2535\u20132543, 2018.<\/jats:p>","DOI":"10.1002\/jbm.b.34070","type":"journal-article","created":{"date-parts":[[2018,1,11]],"date-time":"2018-01-11T02:58:46Z","timestamp":1515639526000},"page":"2535-2543","source":"Crossref","is-referenced-by-count":11,"title":["Preclinical evaluation of melanocyte transplantation by chitosan\u2010based melanocyte spheroid patch to skin prepared by controlled sunburn blistering"],"prefix":"10.1002","volume":"106","author":[{"given":"Sabrina Mai\u2010Yi","family":"Fan","sequence":"first","affiliation":[{"name":"Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Po\u2010Hua","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Yun\u2010Lin Branch National Taiwan University Hospital Doliu Yunlin Taiwan"},{"name":"Department of 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