{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T17:29:31Z","timestamp":1790270971912,"version":"4.1.0"},"reference-count":43,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,10,3]],"date-time":"2018-10-03T00:00:00Z","timestamp":1538524800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000072","name":"National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","award":["R01DE026578"],"award-info":[{"award-number":["R01DE026578"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["K08DE023552"],"award-info":[{"award-number":["K08DE023552"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biomed Mater Res"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Evoked bleeding (EB) clinical procedure, comprising a disinfection step followed by periapical tissue laceration to induce the ingrowth of undifferentiated stem cells from the periodontal ligament and alveolar bone, is currently the only regenerative\u2010based therapeutic approach to treating pulp tissue necrosis in undeveloped (immature) permanent teeth approved in the United States. Yet, the disinfection step using antibiotic\u2010based pastes leads to cytotoxic, warranting a biocompatible strategy to promote root canal disinfection with no or minimal side\u2010effects to maximize the regenerative outcomes. The purpose of this investigation was to develop a tubular three\u2010dimensional (3D) triple antibiotic\u2010eluting construct for intracanal drug delivery. Morphological (scanning electron microscopy), chemical (Fourier transform infrared spectroscopy), and mechanical (tensile testing) characteristics of the polydioxanone\u2010based triple antibiotic\u2010eluting fibers were assessed. The antimicrobial properties of the tubular 3D constructs were determined\n                    <jats:italic>in vitro<\/jats:italic>\n                    and\n                    <jats:italic>in vivo<\/jats:italic>\n                    using an infected (\n                    <jats:italic>Actinomyces naeslundii<\/jats:italic>\n                    ) dentin tooth slice model and a canine method of periapical disease, respectively. The\n                    <jats:italic>in vitro<\/jats:italic>\n                    data indicated significant antimicrobial activity and the ability to eliminate bacterial biofilm inside dentinal tubules.\n                    <jats:italic>In vivo<\/jats:italic>\n                    histological findings demonstrated that, using the EB procedure, the tubular 3D triple antibiotic\u2010eluting construct allowed the formation of an appropriate environment that led to apex closure and the ingrowth of a thin layer of osteodentin\u2010like tissue into the root canal. Taken together, these findings indicate that our novel drug delivery construct is a promising biocompatible disinfection strategy for immature permanent teeth with necrotic pulps. \u00a9 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 1576\u20131586, 2019.\n                  <\/jats:p>","DOI":"10.1002\/jbm.b.34250","type":"journal-article","created":{"date-parts":[[2018,10,5]],"date-time":"2018-10-05T13:10:19Z","timestamp":1538745019000},"page":"1576-1586","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":50,"title":["A novel patient\u2010specific three\u2010dimensional drug delivery construct for regenerative endodontics"],"prefix":"10.1002","volume":"107","author":[{"given":"Marco C.","family":"Bottino","sequence":"first","affiliation":[{"name":"Department of Cariology, Restorative Sciences and Endodontics University of Michigan School of Dentistry  Ann Arbor Michigan 48109"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria T. 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