{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T19:42:51Z","timestamp":1765827771914},"reference-count":47,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2019,2,19]],"date-time":"2019-02-19T00:00:00Z","timestamp":1550534400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100001062","name":"Wallace H. Coulter Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100001062","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biomed Mater Res"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Stress urinary incontinence (SUI), a serious condition which affects ~56% of postmenopausal women, is the involuntary leakage of urine through urethra during physical activity that causes an increase in abdominal pressure. SUI is associated with a decrease in compliance and volume of urethral tissue, likely due to a reduced proteoglycan: collagen ratio in the extracellular matrix and collagen disorganization. Here, we investigated the use of biomimetic proteoglycans (BPGs) to molecularly engineer urethral tissue of New Zealand White rabbits to examine biocompatibility <jats:italic>in vivo<\/jats:italic>. BPG concentrations of 50\u2009mg\/mL (n\u2009=\u20096, 1 week) and 200\u2009mg\/mL (n\u2009=\u20096, 1 week and n\u2009=\u20096, 6 weeks) dissolved in 1\u00d7 phosphate\u2010buffered saline (PBS) were injected transurethrally using a 9 French cystoscope, and were compared to PBS\u2010injected controls (n\u2009=\u20093, 1 week) and non\u2010injected controls (n\u2009=\u20092, 1 week). Urethral compression pressure measurements confirm BPG injections did not modify normal urethral pressure, as intended. Histological assessment demonstrated biological tolerance of BPGs in urethra and no inflammatory response was detected after 1 and 6 weeks compared to non\u2010injected controls. Confocal imaging of fluorescently\u2010labeled BPG injected urethral specimens demonstrated the integration of BPGs into the interstitial connective tissue and confirmed they were still present after 6 weeks. A general decrease of collagen density was exhibited near injection sites which may be due to increased hydration induced by BPGs. Injection of BPGs is a novel approach that demonstrates potential as molecular treatment for SUI and may be able to reverse some of the degenerative tissue changes of individuals affected by this condition. \u00a9 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: 00B: 000\u2013000, 2019. \u00a9 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 2409\u20132418, 2019.<\/jats:p>","DOI":"10.1002\/jbm.b.34334","type":"journal-article","created":{"date-parts":[[2019,2,20]],"date-time":"2019-02-20T08:08:56Z","timestamp":1550650136000},"page":"2409-2418","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["<i>In vivo<\/i> molecular engineering of the urethra for treatment of stress incontinence using novel biomimetic proteoglycans"],"prefix":"10.1002","volume":"107","author":[{"given":"Alicia S.","family":"Kriete","sequence":"first","affiliation":[{"name":"Materials Science and Engineering Drexel University  Philadelphia Pennsylvania 19104"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natasha","family":"Ginzburg","sequence":"additional","affiliation":[{"name":"College of Medicine Drexel University  Philadelphia Pennsylvania 19129"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nima","family":"Shah","sequence":"additional","affiliation":[{"name":"College of Medicine Drexel University  Philadelphia Pennsylvania 19129"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard B.","family":"Huneke","sequence":"additional","affiliation":[{"name":"College of Medicine Drexel University  Philadelphia Pennsylvania 19129"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emily","family":"Reimold","sequence":"additional","affiliation":[{"name":"College of Medicine Drexel University  Philadelphia Pennsylvania 19129"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katsiaryna","family":"Prudnikova","sequence":"additional","affiliation":[{"name":"Materials Science and Engineering Drexel University  Philadelphia Pennsylvania 19104"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Owen","family":"Montgomery","sequence":"additional","affiliation":[{"name":"College of Medicine Drexel University  Philadelphia Pennsylvania 19129"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. Steve","family":"Hou","sequence":"additional","affiliation":[{"name":"College of Medicine Drexel University  Philadelphia Pennsylvania 19129"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Evan R.","family":"Phillips","sequence":"additional","affiliation":[{"name":"Materials Science and Engineering Drexel University  Philadelphia Pennsylvania 19104"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michele S.","family":"Marcolongo","sequence":"additional","affiliation":[{"name":"Materials Science and Engineering Drexel University  Philadelphia Pennsylvania 19104"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,2,19]]},"reference":[{"issue":"1","key":"e_1_2_7_2_1","first-page":"3","article-title":"Bulking agents in the treatment of stress urinary incontinence: History, outcomes, patient populations, and reimbursement profile","volume":"7","author":"Kerr LA","year":"2005","journal-title":"Rev Urol"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1503\/cmaj.060034"},{"issue":"3","key":"e_1_2_7_4_1","first-page":"145","article-title":"Male urinary incontinence: Prevalence, risk factors, and preventive interventions","volume":"11","author":"Shamliyan TA","year":"2009","journal-title":"Rev Urol"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0029-7844(99)00263-X"},{"key":"e_1_2_7_6_1","unstructured":"StreicherL.2014. New Guidelines for Urinary Incontinence But Are They Enough? Midlife Menopause and Beyond. Available from:http:\/\/www.everydayhealth.com\/columns\/lauren-streicher-midlife-menopause-and-beyond\/new-guidelines-urinary-incontinence-are-they-enough\/."},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/SPV.0000000000000159"},{"key":"e_1_2_7_8_1","unstructured":"Healthline.Stress incontinence. Available from:http:\/\/www.healthline.com\/health\/stress-incontinence#Overview1."},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0412.1998.770119.x"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1148\/radiol.2252011347"},{"issue":"5","key":"e_1_2_7_11_1","first-page":"188","article-title":"Pathophysiology of urinary incontinence","volume":"26","author":"Patel AK","year":"2008","journal-title":"Surg Oxford Int Ed"},{"issue":"7","key":"e_1_2_7_12_1","first-page":"307","article-title":"Anatomy of the lower urinary tract","volume":"28","author":"Mangera A","year":"2010","journal-title":"Surg Oxford Int Ed"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.spv.0000080496.16837.3d"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/b136174"},{"issue":"6","key":"e_1_2_7_15_1","first-page":"249","article-title":"Pathophysiology of urinary incontinence","volume":"29","author":"Mangera A","year":"2011","journal-title":"Surg Oxford Int Ed"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.spv.0000117312.27146.f8"},{"key":"e_1_2_7_17_1","volume-title":"Campbell\u2010Walsh Urology","author":"Wein AJ","year":"2011"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/med\/9780199696130.001.0001"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02202011"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1097\/00006250-200003000-00002"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(05)65768-0"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-59745-103-1"},{"key":"e_1_2_7_23_1","volume-title":"Urogynecology and Reconstructive Pelvic Surgery","author":"Walters MD","year":"2014"},{"key":"e_1_2_7_24_1","volume-title":"Immunopharmacology of Joints and Connective Tissue","author":"Davies ME","year":"1994"},{"key":"e_1_2_7_25_1","volume-title":"Molecular cell biology","author":"Lodish HF","year":"2003"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.5357\/koubyou.64.193"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(05)65125-7"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cr.7290106"},{"key":"e_1_2_7_29_1","volume-title":"The Urinary Sphincter","author":"Corcos J","year":"2001"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0110948"},{"key":"e_1_2_7_31_1","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1007\/978-3-031-02577-8","volume-title":"Tissue Engineering of Temporomandibular Joint Cartilage","author":"Athanasiou KA","year":"2009"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.maturitas.2007.09.010"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2018.05.013"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2009.116"},{"key":"e_1_2_7_35_1","volume-title":"Law of Urination: All Mammals Empty their Bladders over the Same Duration","author":"Yang PJ","year":"2013"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1677-55382012000100003"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0112097"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1097\/00006324-198309000-00015"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-78517-7_17"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcm.2006.03.011"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1369\/jhc.2009.953901"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/1282531"},{"key":"e_1_2_7_43_1","volume-title":"Encyclopedia of Cell Biology","author":"Bradshaw RA","year":"2015"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2009.11.013"},{"issue":"2","key":"e_1_2_7_45_1","article-title":"Update on urethral bulking agents for female stress urinary incontinence due to intrinsic sphincter deficiency","volume":"1","author":"Ghoniem G","year":"2014","journal-title":"J Urol Res"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0140851"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00126.2003"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.AOG.0000257074.98122.69"}],"container-title":["Journal of Biomedical Materials Research Part B: Applied Biomaterials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjbm.b.34334","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbm.b.34334","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jbm.b.34334","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbm.b.34334","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,9]],"date-time":"2023-09-09T15:30:53Z","timestamp":1694273453000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbm.b.34334"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,2,19]]},"references-count":47,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2019,10]]}},"alternative-id":["10.1002\/jbm.b.34334"],"URL":"https:\/\/doi.org\/10.1002\/jbm.b.34334","archive":["Portico"],"relation":{},"ISSN":["1552-4973","1552-4981"],"issn-type":[{"value":"1552-4973","type":"print"},{"value":"1552-4981","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,2,19]]},"assertion":[{"value":"2018-08-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-01-13","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-02-19","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}