{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,1]],"date-time":"2024-07-01T23:10:53Z","timestamp":1719875453813},"reference-count":37,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2019,2,18]],"date-time":"2019-02-18T00:00:00Z","timestamp":1550448000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["POIG.02.03.01\u201024\u2010099\/13"],"award-info":[{"award-number":["POIG.02.03.01\u201024\u2010099\/13"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biomed Mater Res"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Water\u2010soluble polymer\u2013methotrexate (MTX) conjugates were obtained via efficient carbodiimide\u2010mediated amidation (<jats:italic>E<\/jats:italic>\u2009=\u200917\u2013100%). Binding abilities between water\u2010soluble V\u2010shaped or star\u2010shaped copolymers and MTX were studied by isothermal titration calorimetry spectroscopic (UV\u2013vis, NMR) and microscopic (scanning electron microscopy and transmission electron microscopy) techniques. The efficiency of the amidation reaction has depended on the amount of pendant amino alcohol groups and zeta potential (ZP) values of polymeric carries. The sizes of aggregates formed by polymer\u2013drug conjugates in water increased with the number of copolymer arms (202\u2013774\u2009nm at 37\u00b0C). Moreover, the conjugates with the high amount of bounded MTX molecules (<jats:italic>n<\/jats:italic><jats:sub>MTX<\/jats:sub>\u2009&gt;\u200978) exhibited negative ZP values. The drug release experiments revealed that the amount of the released MTX depends on pH and can be controlled via shape, topology, and composition of polymeric carrier. Preliminary cytotoxicity studies of V\u2010shaped\u2010MTX conjugate on human immortalized nontumorigenic keratinocyte (HaCaT) cells indicated cytocompatibility of the compound in a wide range of concentrations. The results of our studies have shown that physicochemical and drug release properties of obtained polymer\u2013MTX prodrugs can be tailored <jats:italic>via<\/jats:italic> the structure and the topology of the polymeric carrier. Thus conjugates might find the application in a different type of treatment (cancer or psoriasis therapy) and administration (intravenous, dermal, or pulmonary). \u00a9 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B:2476\u20132487, 2019.<\/jats:p>","DOI":"10.1002\/jbm.b.34338","type":"journal-article","created":{"date-parts":[[2019,2,18]],"date-time":"2019-02-18T09:40:08Z","timestamp":1550482808000},"page":"2476-2487","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Self\u2010assembling water\u2010soluble polymethacrylate\u2013MTX conjugates: The significance of macromolecules architecture on drug conjugation efficiency, the final shape of particles, and drug release"],"prefix":"10.1002","volume":"107","author":[{"given":"Katarzyna","family":"Bernaczek","sequence":"first","affiliation":[{"name":"Department of Physical Chemistry and Technology of Polymers, Faculty of Chemistry Silesian University of Technology  M. 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Strzody 9 Street, 44\u2010100 Gliwice Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zbigniew Jan","family":"Grzywna","sequence":"additional","affiliation":[{"name":"Department of Physical Chemistry and Technology of Polymers, Faculty of Chemistry Silesian University of Technology  M. 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