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Standard treatment for CI is cerclage, which has variable success rates, resulting in a clinical need for alternative treatments. Our objective was to develop an ex vivo model of softened cervical tissue to study an injectable silk\u2010based hydrogel as a novel alternative treatment for CI. Cervical tissue from nonpregnant women was enzymatically treated and characterized to determine tissue hydration, collagen organization, and mechanical properties via unconfined compression. Enzymatic treatment led to an 86\u2009\u00b1\u20097.9% decrease in modulus, which correlated to a decrease in collagen organization as observed by differences in collagen birefringence. The softened tissue was injected with a crosslinked silk\u2010hyaluronic acid composite hydrogel. After injection, the mechanical properties and volume increase of the hydrogel\u2010treated tissue were measured resulting in a 54\u2009\u00b1\u200916% volume increase with minimal effect on tissue mechanical properties. In addition, cervical fibroblasts on silk\u2010hyaluronic acid hydrogels remained viable and exhibited increased proliferation and metabolic activity over 5 days. Overall, this study developed an ex vivo pregnant\u2010like human tissue model to assess cervical augmentation and showed the potential of silk\u2010based hydrogels as an alternative treatment for cervical insufficiency.<\/jats:p>","DOI":"10.1002\/jbm.b.34403","type":"journal-article","created":{"date-parts":[[2019,5,9]],"date-time":"2019-05-09T17:24:52Z","timestamp":1557422692000},"page":"468-474","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Ex vivo pregnant\u2010like tissue model to assess injectable hydrogel for preterm birth prevention"],"prefix":"10.1002","volume":"108","author":[{"given":"Nicole R.","family":"Raia","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering Tufts University  Medford Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie L.","family":"Bakaysa","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and 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