{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T17:42:33Z","timestamp":1759772553696},"reference-count":34,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,7,26]],"date-time":"2019-07-26T00:00:00Z","timestamp":1564099200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["17H04383","17K11778"],"award-info":[{"award-number":["17H04383","17K11778"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biomed Mater Res"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Freeze\u2010dry processing improves the operability and stability of cell\u2010based biomaterials and facilitates sterilization for clinical application. However, there is no established freeze\u2010drying protocol for engineered tissues. Recently, we reported that biomimetic bone tissues can be fabricated using scaffold\u2010free three\u2010dimensional (3D) cell constructs with potential applications as bone graft materials. The purpose of this study was to assess the influence of freeze drying on the morphology and components of 3D cell constructs. Cell constructs freeze dried in phosphate buffered saline (PBS) maintained organic and inorganic components; whereas sodium citrate buffer (SCB)\u2010treated constructs had significantly lower amounts of calcium and bone\u2010related proteins. Alkaline phosphatase (ALP) activity in cell constructs was maintained by freeze drying in 10% sucrose\u2010containing PBS, whereas cell constructs treated with PBS without sucrose or with sucrose\u2010containing SCB showed significant reductions of ALP activity. In this study, we found that sucrose\u2010containing phosphate buffer was suitable for freeze drying to maintain minerals and protein functions within 3D cell constructs, whereas citrate buffer was inappropriate. The insights gained by this study may facilitate the development of novel cell\u2010based biomaterials fabricated by tissue engineering approaches and bone graft biomaterials.<\/jats:p>","DOI":"10.1002\/jbm.b.34448","type":"journal-article","created":{"date-parts":[[2019,7,27]],"date-time":"2019-07-27T03:59:40Z","timestamp":1564199980000},"page":"958-964","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Freeze\u2010dry processing of three\u2010dimensional cell constructs for bone graft materials"],"prefix":"10.1002","volume":"108","author":[{"given":"Jun\u2010Ichi","family":"Sasaki","sequence":"first","affiliation":[{"name":"Department of Biomaterials Science Osaka University Graduate School of Dentistry  Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Itsumi","family":"Yoshimoto","sequence":"additional","affiliation":[{"name":"Department of Biomaterials Science Osaka University Graduate School of Dentistry  Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chihiro","family":"Katata","sequence":"additional","affiliation":[{"name":"Department of Biomaterials Science Osaka University Graduate School of Dentistry  Osaka Japan"},{"name":"Department of Restorative Dentistry and Endodontology Osaka University Graduate School of Dentistry  Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ririko","family":"Tsuboi","sequence":"additional","affiliation":[{"name":"Department of Advanced Functional Materials Science Osaka University Graduate School of Dentistry  Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satoshi","family":"Imazato","sequence":"additional","affiliation":[{"name":"Department of Biomaterials Science Osaka University Graduate School of Dentistry  Osaka Japan"},{"name":"Department of Advanced Functional Materials Science Osaka University Graduate School of Dentistry  Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,7,26]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-409X(00)00144-7"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/SCS.0000000000002548"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2008.09.013"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2006.11.043"},{"key":"e_1_2_7_6_1","first-page":"225","article-title":"Interactions of stabilizing additives with proteins during freeze\u2010thawing and freeze\u2010drying","volume":"74","author":"Carpenter J. 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