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The aim of this study was to compare the therapeutic efficacy of the bilayer poly\u2010D,L\u2010lactide\/poly\u2010\u03b5\u2010caprolactone (PL\u2010PC) scaffold seeded with allogenic mesenchymal stem cells (MSCs) for urethra reconstruction in a rabbit model with conventional urethroplasty employing an autologous buccal mucosa graft (BG). The inner layer of the scaffold based on poly\u2010D,L\u2010lactic acid (PL) was seeded with MSCs, while the outer layer, prepared from poly\u2010\u03b5\u2010caprolactone, protected the surrounding tissues from urine. To track the MSCs in vivo, the latter were labeled with superparamagnetic iron oxide nanoparticles. In rabbits, a dorsal penile defect was reconstructed employing a BG or a PL\u2010PC graft seeded with nanoparticle\u2010labeled MSCs. In the 12\u2010week follow\u2010up period, no complications were detected. Subsequent histological analysis demonstrated biointegration of the PL\u2010PC graft with surrounding urethral tissues. Less fibrosis and inflammatory cell infiltration were observed in the experimental group as compared with the BG group. Nanoparticle\u2010labeled MSCs were detected in the urothelium and muscular layer, co\u2010localizing with the urothelium cytokeratin marker AE1\/AE3, indicating the possibility of MSC differentiation into neo\u2010urothelium. Our results suggest that a bilayer MSCs\u2010seeded scaffold could be efficiently employed for urethroplasty.<\/jats:p>","DOI":"10.1002\/jbm.b.34453","type":"journal-article","created":{"date-parts":[[2019,8,1]],"date-time":"2019-08-01T16:05:23Z","timestamp":1564675523000},"page":"1010-1021","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Urethroplasty with a bilayered poly\u2010D,L\u2010lactide\u2010co\u2010\u03b5\u2010caprolactone scaffold seeded with allogenic mesenchymal stem cells"],"prefix":"10.1002","volume":"108","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7357-1571","authenticated-orcid":false,"given":"Natalia M.","family":"Yudintceva","sequence":"first","affiliation":[{"name":"Institute of Cytology of the Russian Academy of Sciences (RAS)  St. Petersburg Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yulia 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