{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T13:51:47Z","timestamp":1791985907093,"version":"4.4.0"},"reference-count":34,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,8,6]],"date-time":"2019-08-06T00:00:00Z","timestamp":1565049600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100006144","name":"Kerala State Council for Science, Technology and Environment","doi-asserted-by":"publisher","award":["348\/2016\/KSCSTE"],"award-info":[{"award-number":["348\/2016\/KSCSTE"]}],"id":[{"id":"10.13039\/501100006144","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biomed Mater Res"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Treatment with cross\u2010linking agents for stabilizing biomolecules is an integral step during the preparation of many extracellular matrix\u2010based tissue engineering scaffolds from mammalian organs. However, excess cross\u2010linking may cause nonavailability of biomolecules and consequent deterioration of bioinductive properties of the scaffold. The present study considered controlling the extent of cross\u2010linking in a porcine cholecyst extracellular matrix scaffold prepared by a nonenzymatic and nondetergent method, by <jats:italic>ex situ<\/jats:italic> incubation of the source organ in varying concentrations of neutral buffered formaldehyde (10, 4, 1 or 0%; v\/v) for <jats:italic>in situ<\/jats:italic> cross\u2010linking of biomolecules. Reduction of the formaldehyde concentration resulted in an increase in the extent of biodegradation and a decrease in the compactness of the mesh\u2010like surface microarchitecture of the scaffold. Retention of collagen was maximum when treated with 10% neutral buffered formaldehyde without any variation in the content of elastin and sulphated glycosaminoglycans. Although there was a reduction in the quantity of growth factors following the cross\u2010linking, fibroblasts remained viable on the scaffolds. The retention of major biomolecule was maximum and autodigestion was minimum in the scaffold prepared by the <jats:italic>ex situ<\/jats:italic> treatment of cholecyst in 10% neutral buffered formalin and found suitable for preparing the tissue engineering scaffold.<\/jats:p>","DOI":"10.1002\/jbm.b.34457","type":"journal-article","created":{"date-parts":[[2019,8,7]],"date-time":"2019-08-07T04:41:25Z","timestamp":1565152885000},"page":"1057-1067","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Controlled cross\u2010linking of porcine cholecyst extracellular matrix for preparing tissue engineering scaffold"],"prefix":"10.1002","volume":"108","author":[{"given":"Manjula P.","family":"Mony","sequence":"first","affiliation":[{"name":"Division of Experimental Pathology Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology  Thiruvananthapuram 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