{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T17:54:08Z","timestamp":1790704448657,"version":"4.1.0"},"reference-count":42,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,10,23]],"date-time":"2019-10-23T00:00:00Z","timestamp":1571788800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biomed Mater Res"],"published-print":{"date-parts":[[2020,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>More and more scholars regard the lesion of liver cirrhosis as a series of progressive clinical stages. Besides, liver cirrhosis is a late stage of scarring (fibrosis) of the liver, and has long been a common cause of death for the global adult population, thus, the treatment of liver cirrhosis is a key point investigated in the biomedical field. Here, we propose a novel hypothesis; if decellularized liver matrix (DLM) is possible injected into the injurant\u2010induced fiberized liver via the hepatic portal vein to thoroughly repair the liver, it may be an effective therapeutic method for liver fibrosis or even liver cirrhosis. This study mixed rat DLM with gelatin\u2010hydroxyphenylpropionic acid (Glt\u2010HPA) to form a three\u2010dimensional structure to simulate the in vivo liver environment, and cultured the primary rat hepatocytes in it. Afterward, the hepatocytes were treated using D\u2010galactosamine (GaIN), CHCl<jats:sub>3<\/jats:sub>, and CCl<jats:sub>4<\/jats:sub>\u2010containing medium to simulate the toxin\u2010mediated liver fibrosis in vitro. Finally, they were cultured in a DLM\u2010containing medium to observe the viability and functions of the damaged hepatocytes, and the hypothesis of this research was proved, meaning that Glt\u2010HPA\u2010DLM acting on damaged hepatocytes may repair them. Results have shown that Glt\u2010HPA\u2010DLM was effective for hepatocytes culture and repaired injured hepatocytes from GaIN, CHCl<jats:sub>3<\/jats:sub>, and CCl<jats:sub>4<\/jats:sub> (albumin synthesis was increased by 219, 108, and 12%, respectively, whereas relative lactate dehydrogenase activity was reduced by 38, 68, and 67%, after 5\u2009days of culture, separately). This research shows promising effects against hepatic fibrosis and may have potential for liver cirrhosis in vivo.<\/jats:p>","DOI":"10.1002\/jbm.b.34506","type":"journal-article","created":{"date-parts":[[2019,10,23]],"date-time":"2019-10-23T19:28:19Z","timestamp":1571858899000},"page":"1592-1602","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Decellularized liver matrix as substrates for rescue of acute hepatocytes toxicity"],"prefix":"10.1002","volume":"108","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4978-9829","authenticated-orcid":false,"given":"Yung\u2010te","family":"Hou","sequence":"first","affiliation":[{"name":"Department of Biomechatronics Engineering National Taiwan University  Taipei Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shan\u2010hui","family":"Hsu","sequence":"additional","affiliation":[{"name":"Institute of Polymer Science and Engineering National Taiwan University  Taipei Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuang\u2010Min","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Biomechatronics Engineering National Taiwan University  Taipei Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,10,23]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"67","article-title":"Experimental models and hepatotoxic agents used to study hepatoprotective effect of traditional drugs","volume":"2","author":"Ansari S.","year":"2014","journal-title":"International Journal of Advances in Pharmacy Medicine and Bioallied Sciences"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2018.09.014"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-bioeng-071910-124743"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI24282"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/aor.12838"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2008.0172"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.23196"},{"issue":"6","key":"e_1_2_8_9_1","first-page":"603","article-title":"Differential biochemical response of freshly isolated rat hepatocytes to paracetamol, carbon tetrachloride and d\u2010galactosamine toxicity","volume":"35","author":"Chrungoo V. 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