{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T08:21:41Z","timestamp":1791879701217,"version":"4.3.4"},"reference-count":28,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,12,10]],"date-time":"2019-12-10T00:00:00Z","timestamp":1575936000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100009950","name":"Ministry of Education","doi-asserted-by":"publisher","award":["2018R1A6A1A03025582"],"award-info":[{"award-number":["2018R1A6A1A03025582"]}],"id":[{"id":"10.13039\/100009950","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF\u20102019R1D1A3A031033828"],"award-info":[{"award-number":["NRF\u20102019R1D1A3A031033828"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biomed Mater Res"],"published-print":{"date-parts":[[2020,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Nanocrystalline hydroxyapatite (HAp) was synthesized from biowaste eggshells through sonication followed by the heat treatment. Calcium oxide as a precursor moiety for the synthesis of HAp was obtained through the heat treatment of eggshells at 900\u00b0C for 3\u2009hr. The prepared HAp was characterized by Fourier\u2010transform infrared spectroscopy (FTIR), X\u2010ray powder diffraction (XRD), and scanning electron microscopy (SEM). The appearance of the FTIR absorption peaks in between at 516\u20131031 and 3,636\u2009cm<jats:sup>\u22121<\/jats:sup> shows phosphate and hydroxyl groups in prepared HAp, respectively. The XRD\u2010patterns indicate the formation of HAp started within 5 min of sonication. The SEM morphologies suggested that the synthesized HAp was highly crystalline and compact. We tested the elemental analysis of the synthesized HAp through X\u2010ray fluorescence spectroscopy and inductively coupled plasma mass spectroscopy. The higher Ca\/P ratio has observed in heat\u2010treated HAp. These results show that heat treatment facilitates the formation of highly crystalline and compact HAp. Cytotoxicity and osteogenic potential of human mesenchymal stem cells (hMSCs) were also evaluated in the presence of HAp. No significant cytotoxicity was noted in the presence of HAp, suggested their biocompatibility. Enhanced osteogenesis of hMSCs occurred with HAp powder, confirming the feasibility in the treatment of osteogenesis. Thus, synthesized HAp has the potential to use a biomaterial in tissue engineering applications for bone tissues.<\/jats:p>","DOI":"10.1002\/jbm.b.34536","type":"journal-article","created":{"date-parts":[[2019,12,10]],"date-time":"2019-12-10T13:03:07Z","timestamp":1575982987000},"page":"1953-1960","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Osteogenic potential of human mesenchymal stem cells on eggshells\u2010derived hydroxyapatite nanoparticles for tissue engineering"],"prefix":"10.1002","volume":"108","author":[{"given":"Dinesh K.","family":"Patel","sequence":"first","affiliation":[{"name":"The Institute of Forest Science, Kangwon National University  Chuncheon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Stomatology Affiliated Hospital of Yanbian University  Yanji 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