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Sterilization by ionizing radiation is the most effective method of minimizing the bioburden of bone allografts; however, radiation causes chain scission of collagen, resulting in the reduction of the allografts\u2019 mechanical strength. In this study, we doped bone allografts with vitamin E as radioprotectant using a novel two\u2010step process to protect the collagen architecture against radiation damage and to preserve the mechanical strength of the construct. In addition, combining the radioprotectant with a cross\u2010linking agent further minimized collagen degradation and further preserved the mechanical strength of the allografts. Both vitamin E and combined vitamin E\/genipin\u2010treated allograft were less cytotoxic to both osteoblasts and osteoclasts when compared to irradiated\u2010only allografts. Host bone\u2010allograft unionization was faster in a rat calvaria defect model with vitamin E\u2010treated and combined vitamin E and genipin\u2010treated allograft when compare to irradiated\u2010only allografts. This method can enable the efficient and uniform radioprotective treatment of bone allograft of desired shapes for sterilization with improved mechanical strength and biointegration.<\/jats:p>","DOI":"10.1002\/jbm.b.34569","type":"journal-article","created":{"date-parts":[[2020,1,24]],"date-time":"2020-01-24T09:10:02Z","timestamp":1579857002000},"page":"2354-2367","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Radioprotection and cross\u2010linking of allograft bone in the presence of vitamin E"],"prefix":"10.1002","volume":"108","author":[{"given":"Jeremy V.","family":"Suhardi","sequence":"first","affiliation":[{"name":"Harris Orthopedic Laboratory Massachusetts General Hospital  Boston Massachusetts"},{"name":"Department of Medical Engineering and Medical Physics Massachusetts Institute of Technology  Boston Massachusetts"},{"name":"Department of Orthopaedic Surgery Harvard Medical School  Boston Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David F. 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