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Cytotoxicity, cell death, and genotoxicity tests were carried out with extract samples of 24\u2010hr and 7\u2010days. The release of Ag\n                    <jats:sup>+<\/jats:sup>\n                    and V\n                    <jats:sup>4+<\/jats:sup>\n                    \/V\n                    <jats:sup>5+<\/jats:sup>\n                    was evaluated. Cytotoxicity in HGF was caused by AH Plus (AP) with 5 and 10% of AgVO\n                    <jats:sub>3<\/jats:sub>\n                    (83.84 and 67.49% cell viability, respectively) with 24\u2010hr extract (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05), as well as all concentrations of AP with 7\u2010days extract (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05 \u2013AP 0%\u00a0=\u00a073.17%; AP 2.5%\u00a0=\u00a075.07%; AP 5%\u00a0=\u00a070.62%; AP 10%\u00a0=\u00a068.46% cell viability). The commercial sealers Sealer 26 (S26) and Endomethasone N (EN) were cytotoxic (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05 \u2013 S26 0%\u00a0=\u00a034.81%; EN 0%\u00a0=\u00a020.99% cell viability with 7\u2010days extract). AP 10% with 7\u2010days extract induced 32% apoptotic cells in HGF (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). Genotoxic effect was not observed. The AP groups released more Ag\n                    <jats:sup>+<\/jats:sup>\n                    , while S26 and EN released more V\n                    <jats:sup>4+<\/jats:sup>\n                    \/V\n                    <jats:sup>5+<\/jats:sup>\n                    in 24\u2009hr. The Ag\n                    <jats:sup>+<\/jats:sup>\n                    can be cytotoxic. In conclusion, the cytotoxicity caused to HGF can be attributed by the commercial sealers and enhanced by incorporation of AgVO\n                    <jats:sub>3<\/jats:sub>\n                    , was not observed genotoxic effect, and apoptosis was induced only by AH Plus 10% 7\u2010days extract. Ag\n                    <jats:sup>+<\/jats:sup>\n                    can influence cell viability.\n                  <\/jats:p>","DOI":"10.1002\/jbm.b.34798","type":"journal-article","created":{"date-parts":[[2021,1,20]],"date-time":"2021-01-20T04:21:55Z","timestamp":1611116515000},"page":"1380-1388","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Cytotoxic and genotoxic effects in human gingival fibroblast and ions release of endodontic sealers incorporated with nanostructured silver vanadate"],"prefix":"10.1002","volume":"109","author":[{"given":"Ana B. V.","family":"Teixeira","sequence":"first","affiliation":[{"name":"Departament of Dental Materials and Prosthesis Ribeir\u00e3o Preto School of Dentistry, University of S\u00e3o Paulo  Ribeir\u00e3o Preto Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nat\u00e1lia C. S.","family":"Moreira","sequence":"additional","affiliation":[{"name":"Departament of Genetics Ribeir\u00e3o Preto Medical School, University of S\u00e3o Paulo  Ribeir\u00e3o Preto Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catarina S.","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Departament of Genetics Ribeir\u00e3o Preto Medical School, University of S\u00e3o Paulo  Ribeir\u00e3o Preto Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco A.","family":"Schiavon","sequence":"additional","affiliation":[{"name":"Departament of Natural Sciences Federal University of S\u00e3o Jo\u00e3o Del\u2010Rei  S\u00e3o Jo\u00e3o Del\u2010Rei Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oswaldo L.","family":"Alves","sequence":"additional","affiliation":[{"name":"Laboratory of Solid State Chemistry Institute of Chemistry, University of Campinas (Unicamp)  Campinas 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