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However, these reagents have practical constraints, including limited shelf\u2010life and require constant refrigeration. To address these issues, we explore the effects of rapid freeze\u2010drying conditions with trehalose cryoprotectant (0.1\u20131\u00a0M concentrations) on human RBCs and storage of freeze\u2010dried RBCs (FDRBCs) at room temperature (RT) for up to 12\u2009months. We report that rapid freeze\u2010drying of RBCs for 2.5\u00a0hr with 0.5\u00a0M trehalose achieves recoverable cells with near\u2010normal morphological shape, although size\u2010reduced. The FDRBCs are metabolically active and functional in antibody\u2010agglutination tests by the column agglutination test (CAT) for ABO and Rhesus\u2010D blood group antigens. Expression of the Duffy blood group protein (CD234) decreases by 50% after freeze\u2010drying RBCs. The initial recovery rate is \u226425%; however, 43% of these FDRBCs are still recoverable after RT storage for 12\u2009months. In this proof\u2010of\u2010principle study, we show that rapid freeze\u2010drying can stabilize RBCs. Further refinements to improve the recovery rate and preservation of antigenic epitopes will make FDRBCs a practical alternative source of reagent RBCs for pre\u2010transfusion alloantibody identification.<\/jats:p>","DOI":"10.1002\/jbm.b.34825","type":"journal-article","created":{"date-parts":[[2021,3,11]],"date-time":"2021-03-11T05:20:26Z","timestamp":1615440026000},"page":"1689-1697","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Rapidly freeze\u2010dried human red blood cells for pre\u2010transfusion alloantibody testing reagents"],"prefix":"10.1002","volume":"109","author":[{"given":"Diana","family":"Alves","sequence":"first","affiliation":[{"name":"Bioresource Processing Research Institute of Australia (BioPRIA), Department of Chemical Engineering Monash University  Clayton Victoria 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