{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T12:45:04Z","timestamp":1791895504517,"version":"4.3.4"},"reference-count":39,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,1,21]],"date-time":"2022-01-21T00:00:00Z","timestamp":1642723200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100014718","name":"Innovative Research Group Project of the National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81671529"],"award-info":[{"award-number":["81671529"]}],"id":[{"id":"10.13039\/100014718","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biomed Mater Res"],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In tracheal tissue engineering, the construction of tracheal scaffolds with adequate biodegradable mechanical capacity and biological functions that mimic the structure of a natural trachea is challenging. To explore the feasibility of preparing biomimetic degradable scaffolds with C\u2010type cartilage rings and an inner tracheal wall of polycaprolactone and silk fibroin. A mold was made according to the diameter of a rabbit trachea, and a silk fibroin tube and polycaprolactone ring attached to the tube were obtained by solution casting. The ring was fixed to the tube at a specific spacing using electrostatic spinning technology to construct a biomimetic tracheal scaffold; its porous structure was observed by scanning electron microscopy, its degradation properties were determined by in vitro enzymatic hydrolysis and its mechanical properties were obtained by pressure testing. The composite scaffold was transplanted subcutaneously into a rabbit model, and the scaffold was taken at 1, 2, and 4\u2009weeks after surgery for sectioning to observe pre\u2010vascularization. The Medical Ethics Committee of Guangdong Provincial People's Hospital approved the study. The general view of the biomimetic scaffold: the polycaprolactone ring was fixed firmly on the outer wall of the silk fibroin tube; the two corresponded in size, and they fitted closely. The surface of the polycaprolactone ring was smooth and dense, while the surface of the silk fibroin tube could be seen as a uniform porous structure. Scanning electron microscopy showed that the surface and profile of the fibroin tube had a uniform pore size and distribution. The pores were connected to form a network. In vitro, enzymatic hydrolysis experiments confirmed that the fibroin was degraded easily, with most being degraded at the end of week 1. The degradation slowed at 2, 3, and 4\u2009weeks, while the degradation of polycaprolactone was extremely slow. A compression test showed that the compressive resistance of the silk fibroin\u2013polycaprolactone biomimetic scaffolds was much better than that of the rabbit trachea at close thickness. In the tissue staining experiments, as the material degraded, fibrous tissues and blood vessels grew to replace the material, allowing the scaffold to obtain a blood supply and better mechanical properties. A quantitative analysis of CD31 showed that the results for the vascularization of the scaffold were better at 4\u2009weeks than at 2\u2009weeks following subcutaneous grafting (<jats:italic>P<\/jats:italic>\u2009&lt;\u2009.05). The results confirmed that it is feasible to prepare porous, degradable silk fibroin\u2013polycaprolactone biomimetic scaffolds with good mechanical properties and epithelial biological functions by mold casting.<\/jats:p>","DOI":"10.1002\/jbm.b.35000","type":"journal-article","created":{"date-parts":[[2022,1,21]],"date-time":"2022-01-21T15:01:55Z","timestamp":1642777315000},"page":"1292-1305","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Preparation and evaluation of a silk fibroin\u2013polycaprolactone biodegradable biomimetic tracheal scaffold"],"prefix":"10.1002","volume":"110","author":[{"given":"Cai\u2010Sheng","family":"Liu","sequence":"first","affiliation":[{"name":"School of Medicine South China University of Technology  Guangzhou China"},{"name":"Department of Neonatology, Guangdong Provincial People's Hospital Guangdong Academy of Medical Sciences  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo\u2010Wen","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Child Healthcare Guangzhou Women and Children's Medical Center  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shao\u2010Ru","family":"He","sequence":"additional","affiliation":[{"name":"School of Medicine South China University of Technology  Guangzhou China"},{"name":"Department of Neonatology, Guangdong Provincial People's Hospital Guangdong Academy of Medical Sciences  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu\u2010Mei","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Neonatology, Guangdong Provincial People's Hospital Guangdong Academy of Medical Sciences  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Neonatology, Guangdong Provincial People's Hospital Guangdong Academy of Medical Sciences  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan\u2010Ling","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Neonatology, Guangdong Provincial People's Hospital Guangdong Academy of Medical Sciences  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi\u2010Ye","family":"Yao","sequence":"additional","affiliation":[{"name":"Department of Neonatology, Guangdong Provincial People's Hospital Guangdong Academy of Medical Sciences  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min\u2010Qiao","family":"Jian","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Sun Yat\u2010Sen Memorial Hospital Sun Yat\u2010Sen University  Guangzhou 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