{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T16:41:34Z","timestamp":1789231294487,"version":"build-2803163510"},"reference-count":31,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,8,17]],"date-time":"2022-08-17T00:00:00Z","timestamp":1660694400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biomed Mater Res"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The treatment of bone regeneration failures has been constantly improved with the study of new biomaterials. Techgraft\u00ae is a collagen membrane derived from bovine pericardium, which has been shown to have biocompatibility and effectiveness in tissue repair. However, its use in orthopedics has not yet been evaluated. Therefore, the purpose of this study was to characterize a bovine pericardium collagen membrane and evaluate the effects of its use in the regeneration of a bone defect in rat tibia. Scanning electron microscopy, atomic force microscopy, weight lost and water uptake tests, and mechanical test were performed. Afterwards, the membrane was tested in an experimental study, using 12 male Sprague Dawley rats. A bone defect was surgically made in tibiae of animals, which were assigned to two groups (<jats:italic>n<\/jats:italic>\u00a0=\u00a06): bone defect treated with collagen membrane (TG) and bone defect without treatment (CONT). Then, tibiae were submitted to micro\u2010CT. The membranes preserved their natural collagen characteristics, presenting great strength, high water absorption, hydrophilicity, and almost complete dissolution in 30\u2009days. In the experimental study, the membrane enhanced the growth of bone tissue in contact with its surface. A higher bone volume and trabeculae number and less trabecular space was observed in bone defects of the membrane group compared to the control group at 21\u2009days. In conclusion, the Techgraft membrane seems to have favorable characteristics for treatment of long bone repair.<\/jats:p>","DOI":"10.1002\/jbm.b.35148","type":"journal-article","created":{"date-parts":[[2022,8,17]],"date-time":"2022-08-17T09:19:49Z","timestamp":1660727989000},"page":"261-270","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Collagen membrane from bovine pericardium for treatment of long bone defect"],"prefix":"10.1002","volume":"111","author":[{"given":"J\u00e9ssica S.","family":"Yamanaka","sequence":"first","affiliation":[{"name":"Departamento de Ortopedia e Anestesiologia. Faculdade de Medicina de Ribeir\u00e3o Preto Universidade de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Clara","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Departamento de Ortopedia e Anestesiologia. Faculdade de Medicina de Ribeir\u00e3o Preto Universidade de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Raquel","family":"Bastos","sequence":"additional","affiliation":[{"name":"3B's Research Group, I3Bs \u2010 Research Institute on Biomaterials, Biodegradables and Biomimetics of University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark \u2013 Parque de Ci\u00eancia e Tecnologia, Zona Industrial da Gandra  Barco \u2010 Guimar\u00e3es Portugal"},{"name":"ICVS\/3B's \u2013 PT Government Associate Laboratory  Braga Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emanuel M.","family":"Fernandes","sequence":"additional","affiliation":[{"name":"3B's Research Group, I3Bs \u2010 Research Institute on Biomaterials, Biodegradables and Biomimetics of University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark \u2013 Parque de Ci\u00eancia e Tecnologia, Zona Industrial da Gandra  Barco \u2010 Guimar\u00e3es Portugal"},{"name":"ICVS\/3B's \u2013 PT Government Associate Laboratory  Braga Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui L.","family":"Reis","sequence":"additional","affiliation":[{"name":"3B's Research Group, I3Bs \u2010 Research Institute on Biomaterials, Biodegradables and Biomimetics of University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark \u2013 Parque de Ci\u00eancia e Tecnologia, Zona Industrial da Gandra  Barco \u2010 Guimar\u00e3es Portugal"},{"name":"ICVS\/3B's \u2013 PT Government Associate Laboratory  Braga Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vitor M.","family":"Correlo","sequence":"additional","affiliation":[{"name":"3B's Research Group, I3Bs \u2010 Research Institute on Biomaterials, Biodegradables and Biomimetics of University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark \u2013 Parque de Ci\u00eancia e Tecnologia, Zona Industrial da Gandra  Barco \u2010 Guimar\u00e3es Portugal"},{"name":"ICVS\/3B's \u2013 PT Government Associate Laboratory  Braga Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ant\u00f4nio Carlos","family":"Shimano","sequence":"additional","affiliation":[{"name":"Departamento de Ortopedia e Anestesiologia. Faculdade de Medicina de Ribeir\u00e3o Preto Universidade de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,8,17]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2012.08.010"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(00)00102-2"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10856-013-4980-8"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0102-76382005000100007"},{"key":"e_1_2_9_6_1","first-page":"1","article-title":"Process for the extraction of collagen from tissues with aqueous organic solvent in alkaline media","volume":"1","author":"Goissis G","year":"1994","journal-title":"Braz Pat Ind Prop"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-1383(13)70007-X"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/09205063.2019.1627653"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(01)00126-0"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1525-1594.2003.07252.x"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/bm0001188"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmbbm.2015.05.023"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/mabi.200600233"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1088\/1748-6041\/4\/4\/045002"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/521547"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2015.10.005"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/eos.12364"},{"issue":"109959","key":"e_1_2_9_18_1","first-page":"1","article-title":"Fabrication of collagen membranes with different intrafibrillar mineralization degree as a potential use for GBR","volume":"104","author":"Wang J","year":"2019","journal-title":"Mater Sci Eng C"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/mabi.200600063"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/5163603"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13233-014-2138-2"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0039-1693751"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2016.05.021"},{"issue":"3","key":"e_1_2_9_24_1","first-page":"12","article-title":"Biocompatibility of anionic collagen matrices and its influence on the orientation of cellular growth","volume":"10","author":"Buchaim RL","year":"2007","journal-title":"Ci\u00eancia Odontol\u00f3gica Bras"},{"key":"e_1_2_9_25_1","first-page":"1","article-title":"Molecular pathways of cell\u2010mediated degradation of fibrillar collagen","volume":"1","author":"Sprangers S","year":"2017","journal-title":"Matrix Biol"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2016.01.034"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.injury.2015.03.039"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2011.100466"},{"issue":"2","key":"e_1_2_9_29_1","first-page":"225","article-title":"Periodontal regeneration of dehisence defects using a modified perforated collagen membrane. A comparative experimental study. Futur","volume":"4","author":"Fahmy RA","year":"2018","journal-title":"Dent J"},{"issue":"12","key":"e_1_2_9_30_1","first-page":"1","article-title":"Strontium\u2010doped calcium phosphate and hydroxyapatite granules promote different inflammatory and bone remodelling responses in normal and ovariectomised rats","volume":"8","author":"Cardemi C","year":"2013","journal-title":"PLoS One"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2017.09.005"},{"issue":"1","key":"e_1_2_9_32_1","first-page":"73","article-title":"Cultura de osteoblastos sobre membranas de col\u00e1geno poliani\u00f4nico: avalia\u00e7\u00e3o preliminar do potencial de indu\u00e7\u00e3o da forma\u00e7\u00e3o de tecido \u00f3sseo visando repara\u00e7\u00e3o tecidual","volume":"1","author":"Cunha MR","year":"2005","journal-title":"Bol Med Veterin\u00e1ria \u2010 UNIPINHAL"}],"container-title":["Journal of Biomedical Materials Research Part B: Applied Biomaterials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbm.b.35148","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jbm.b.35148","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbm.b.35148","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T00:16:14Z","timestamp":1692576974000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbm.b.35148"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8,17]]},"references-count":31,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2023,2]]}},"alternative-id":["10.1002\/jbm.b.35148"],"URL":"https:\/\/doi.org\/10.1002\/jbm.b.35148","archive":["Portico"],"relation":{},"ISSN":["1552-4973","1552-4981"],"issn-type":[{"value":"1552-4973","type":"print"},{"value":"1552-4981","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,8,17]]},"assertion":[{"value":"2020-07-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-08-01","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-08-17","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}