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Integrin\u2010targeted LTT has been developed recently for angiogenesis\u2010related diseases. However, the clinical success is based on the optimal design of the nanoparticles for inducing receptor clustering within the cell membrane. The current study focused on determining the surface density of Ser\u2010Asp\u2010Val containing anti\u2010integrin heptapeptide on poly (ethylene glycol)\u2010<jats:italic>b<\/jats:italic>\u2010poly(propylene sulfide) micelles (MC) required for anti\u2010angiogenic effects on HUVECs. Varying peptide density on PEG\u2010<jats:italic>b<\/jats:italic>\u2010PPS\/Pep\u2010PA MCs (Pep\u2010PA\u2010Peptide\u2010palmitoleic acid) was used in comparison to a random peptide (SGV) and cRGD (cyclic\u2010Arginine\u2010Glycine\u2010Aspartic acid) construct at 5%\u2010density on MCs. Immunocytochemistry using CD51\/CD31 antibody was performed to study the integrin blocking by MCs. In addition, the expression of VWF and PECAM\u20101, cell migration and tube formation was evaluated in the presence of PEG\u2010<jats:italic>b<\/jats:italic>\u2010PPS\/Pep\u2010PA MCs. The results show PEG\u2010<jats:italic>b<\/jats:italic>\u2010PPS\/SDV\u2010PA MCs with 5%\u2010peptide density to achieve significantly higher \u03b1v\u03b23 blocking compared to random peptide as well as cRGD. In addition, \u03b1v\u03b23 blocking via MCs further reduced the expression of vWF and PECAM\u20101 angiogenesis protein expression in HUVECs. Although a significant level of integrin blocking was observed for 1%\u2010peptide density on MCs, the cell migration and tube formation were not significantly affected. In conclusion, the results of this study demonstrate that the peptide surface density on PEG\u2010<jats:italic>b<\/jats:italic>\u2010PPS\/Pep\u2010PA MCs has a significant impact in integrin blocking as well as inhibiting angiogenesis during LTT. The outcomes of this study provides insight into the design of ligand targeted nanocarriers for various disease conditions.<\/jats:p>","DOI":"10.1002\/jbm.b.35154","type":"journal-article","created":{"date-parts":[[2022,8,30]],"date-time":"2022-08-30T08:07:39Z","timestamp":1661846859000},"page":"343-353","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["In vitro assessment of varying peptide surface density on the suppression of angiogenesis by micelles displaying \u03b1v\u03b23 blocking peptides"],"prefix":"10.1002","volume":"111","author":[{"given":"Neha Phani","family":"Bhushan","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences University of Illinois College of Medicine at Rockford  Rockford Illinois USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Trevor","family":"Stack","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering 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