{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T15:16:54Z","timestamp":1747149414435,"version":"3.40.5"},"reference-count":47,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,9,1]],"date-time":"2022-09-01T00:00:00Z","timestamp":1661990400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biomed Mater Res"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Calcium sulfate, an injectable and biodegradable bone\u2010void filler, is widely used in orthopedic surgery. Based on clinical experience, bone\u2010defect substitutes can also serve as vehicles for the delivery of drugs, for example, antibiotics, to prevent or to treat infections such as osteomyelitis. However, antibiotic additions change the characteristics of calcium sulfate cement. Moreover, high\u2010dose antibiotics may also be toxic to bony tissues. Accordingly, cefazolin at varying weight ratios was added to calcium sulfate samples and characterized in vitro. The results revealed that cefazolin changed the hydration reaction and prolonged the initial setting times of calcium sulfate bone cement. For the crystalline structure identification, X\u2010ray diffractometer revealed that cefazolin additive resulted in the decrease of peak intensity corresponding to calcium sulfate dihydrate which implying incomplete phase conversion of calcium sulfate hemihydrate. In addition, scanning electron microscope inspection exhibited cefazolin changed the morphology and size of the crystals greatly. A relatively higher amount of cefazolin additive caused a faster degradation and a lower compressive strength of calcium sulfate compared with those of uploaded samples. Furthermore, the extract of cefazolin\u2010impregnated calcium sulfate impaired cell viability, and caused the death of osteoblast\u2010like cells. The results of this study revealed that the cefazolin additives prolonged setting time, impaired mechanical strength, accelerated degradation, and caused cytotoxicity of the calcium sulfate bone\u2010void filler. The aforementioned concerns should be considered during intra\u2010operative applications.<\/jats:p>","DOI":"10.1002\/jbm.b.35157","type":"journal-article","created":{"date-parts":[[2022,9,1]],"date-time":"2022-09-01T13:37:39Z","timestamp":1662039459000},"page":"382-391","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["An assessment of physical properties and the viability of osteoblast\u2010like cells of cefazolin\u2010impregnated calcium sulfate bone\u2010void filler"],"prefix":"10.1002","volume":"111","author":[{"given":"Chih\u2010Yung","family":"Chiang","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering, College of Engineering and College of Medicine National Taiwan University  Taipei Taiwan"},{"name":"Department of Orthopedics En Chu Kong Hospital  New Taipei City Taiwan"},{"name":"Department of Orthopaedic Surgery National Taiwan University Hospital  Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei\u2010Chia","family":"Chang","sequence":"additional","affiliation":[{"name":"School of Dental Technology, College of Oral Medicine Taipei Medical University  Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei\u2010Min","family":"Chang","sequence":"additional","affiliation":[{"name":"School of Oral Hygiene, College of Oral Medicine Taipei Medical University  Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yin\u2010Chuan","family":"Shih","sequence":"additional","affiliation":[{"name":"Department of Orthopedics En Chu Kong Hospital  New Taipei City Taiwan"},{"name":"Department of Orthopaedic Surgery National Taiwan University Hospital  Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng\u2010Huei","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, College of Engineering and College of Medicine National Taiwan University  Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chang\u2010Chin","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Orthopedics En Chu Kong Hospital  New Taipei City Taiwan"},{"name":"Department of Orthopaedic Surgery National Taiwan University Hospital  Taipei Taiwan"},{"name":"Department of Biomedical Engineering Yuanpei University of Medical Technology  Hsinchu Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0979-4463","authenticated-orcid":false,"given":"Kai\u2010Chiang","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Dental Technology, College of Oral Medicine Taipei Medical University  Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,9]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_9_2_1","DOI":"10.1097\/00001665-200011040-00009"},{"doi-asserted-by":"publisher","key":"e_1_2_9_3_1","DOI":"10.1016\/j.cden.2019.12.006"},{"doi-asserted-by":"publisher","key":"e_1_2_9_4_1","DOI":"10.1007\/978-3-030-19962-3_15"},{"doi-asserted-by":"publisher","key":"e_1_2_9_5_1","DOI":"10.1097\/00003086-200101000-00008"},{"doi-asserted-by":"publisher","key":"e_1_2_9_6_1","DOI":"10.1002\/jbm.b.31269"},{"doi-asserted-by":"publisher","key":"e_1_2_9_7_1","DOI":"10.18103\/mra.v8i11.2283"},{"issue":"1","key":"e_1_2_9_8_1","doi-asserted-by":"crossref","first-page":"s131","DOI":"10.3928\/0147-7447-20040102-11","article-title":"Treatment of benign bone lesions with an injectable calcium sulfate\u2010based bone graft substitute","volume":"27","author":"Kelly CM","year":"2004","journal-title":"Orthopedics"},{"doi-asserted-by":"publisher","key":"e_1_2_9_9_1","DOI":"10.1002\/jbm.b.30880"},{"doi-asserted-by":"publisher","key":"e_1_2_9_10_1","DOI":"10.1088\/1748-6041\/11\/2\/025012"},{"doi-asserted-by":"publisher","key":"e_1_2_9_11_1","DOI":"10.1002\/jor.25135"},{"doi-asserted-by":"publisher","key":"e_1_2_9_12_1","DOI":"10.1002\/jbm.b.33181"},{"doi-asserted-by":"publisher","key":"e_1_2_9_13_1","DOI":"10.1097\/01.ASW.0000407647.12832.6c"},{"doi-asserted-by":"publisher","key":"e_1_2_9_14_1","DOI":"10.1016\/j.foot.2015.07.002"},{"doi-asserted-by":"publisher","key":"e_1_2_9_15_1","DOI":"10.1097\/01.blo.0000181144.01306.b0"},{"doi-asserted-by":"publisher","key":"e_1_2_9_16_1","DOI":"10.3928\/01477447-20090501-03"},{"doi-asserted-by":"publisher","key":"e_1_2_9_17_1","DOI":"10.1097\/BPB.0000000000000517"},{"doi-asserted-by":"publisher","key":"e_1_2_9_18_1","DOI":"10.7150\/jbji.41743"},{"doi-asserted-by":"publisher","key":"e_1_2_9_19_1","DOI":"10.1155\/2018\/8065141"},{"doi-asserted-by":"publisher","key":"e_1_2_9_20_1","DOI":"10.1093\/ofid\/ofaa650"},{"doi-asserted-by":"publisher","key":"e_1_2_9_21_1","DOI":"10.1002\/jbm.b.33815"},{"doi-asserted-by":"publisher","key":"e_1_2_9_22_1","DOI":"10.1016\/j.procbio.2014.09.009"},{"doi-asserted-by":"publisher","key":"e_1_2_9_23_1","DOI":"10.1002\/jor.21343"},{"issue":"4","key":"e_1_2_9_24_1","first-page":"872","article-title":"Thermal stability assessment of antibiotics in moderate temperature and subcriticalwater using a pressurized dynamic flow\u2010through system","volume":"11","author":"Svahn O","year":"2015","journal-title":"J Innov Appl Stud"},{"doi-asserted-by":"publisher","key":"e_1_2_9_25_1","DOI":"10.1016\/j.porgcoat.2021.106385"},{"key":"e_1_2_9_26_1","first-page":"55","article-title":"Kirby\u2010Bauer disk diffusion susceptibility test protocol","volume":"15","author":"Hudzicki J","year":"2009","journal-title":"Am Soc Microbiol"},{"doi-asserted-by":"publisher","key":"e_1_2_9_27_1","DOI":"10.7150\/jbji.38373"},{"doi-asserted-by":"publisher","key":"e_1_2_9_28_1","DOI":"10.5005\/jp-journals-10017-1027"},{"doi-asserted-by":"publisher","key":"e_1_2_9_29_1","DOI":"10.1007\/s11420-011-9259-3"},{"doi-asserted-by":"publisher","key":"e_1_2_9_30_1","DOI":"10.1302\/2046-3758.65.BJR-2017-0276.R1"},{"doi-asserted-by":"publisher","key":"e_1_2_9_31_1","DOI":"10.1016\/S0169-1317(99)00005-8"},{"issue":"2","key":"e_1_2_9_32_1","first-page":"29","article-title":"Methods for qualitative analysis of cefazolin sodium raw material and pharmaceutical product","volume":"3","author":"Pedroso TM","year":"2013","journal-title":"Phys Chem"},{"doi-asserted-by":"publisher","key":"e_1_2_9_33_1","DOI":"10.1016\/j.biomaterials.2006.01.017"},{"doi-asserted-by":"publisher","key":"e_1_2_9_34_1","DOI":"10.1021\/acs.cgd.6b01441"},{"doi-asserted-by":"publisher","key":"e_1_2_9_35_1","DOI":"10.1016\/j.pcrysgrow.2007.01.002"},{"doi-asserted-by":"publisher","key":"e_1_2_9_36_1","DOI":"10.1615\/JLongTermEffMedImplants.v15.i6.30"},{"doi-asserted-by":"publisher","key":"e_1_2_9_37_1","DOI":"10.1002\/jbm.a.30283"},{"doi-asserted-by":"publisher","key":"e_1_2_9_38_1","DOI":"10.1163\/156856207781367710"},{"doi-asserted-by":"publisher","key":"e_1_2_9_39_1","DOI":"10.1016\/0021-9797(73)90318-4"},{"doi-asserted-by":"publisher","key":"e_1_2_9_40_1","DOI":"10.1002\/jbm.b.33045"},{"doi-asserted-by":"publisher","key":"e_1_2_9_41_1","DOI":"10.1016\/S0025-6196(12)61731-5"},{"doi-asserted-by":"publisher","key":"e_1_2_9_42_1","DOI":"10.1177\/0885328216663392"},{"doi-asserted-by":"publisher","key":"e_1_2_9_43_1","DOI":"10.1016\/j.biomaterials.2009.01.008"},{"unstructured":"ChanH MijaresD RicciJL.In vitro dissolution of calcium sulfate: evidence of bioactivity. In: transactions \u2010 Seventh World Biomaterials Congress 2004. Sydney;2004. p.627.","key":"e_1_2_9_44_1"},{"doi-asserted-by":"publisher","key":"e_1_2_9_45_1","DOI":"10.1021\/la104876w"},{"doi-asserted-by":"publisher","key":"e_1_2_9_46_1","DOI":"10.1179\/joc.2005.17.2.174"},{"doi-asserted-by":"publisher","key":"e_1_2_9_47_1","DOI":"10.1007\/s10856-012-4704-5"},{"doi-asserted-by":"publisher","key":"e_1_2_9_48_1","DOI":"10.1016\/j.biomaterials.2003.09.037"}],"container-title":["Journal of Biomedical Materials Research Part B: Applied Biomaterials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbm.b.35157","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jbm.b.35157","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbm.b.35157","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T00:15:46Z","timestamp":1692576946000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbm.b.35157"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9]]},"references-count":47,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2023,2]]}},"alternative-id":["10.1002\/jbm.b.35157"],"URL":"https:\/\/doi.org\/10.1002\/jbm.b.35157","archive":["Portico"],"relation":{},"ISSN":["1552-4973","1552-4981"],"issn-type":[{"type":"print","value":"1552-4973"},{"type":"electronic","value":"1552-4981"}],"subject":[],"published":{"date-parts":[[2022,9]]},"assertion":[{"value":"2022-06-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-08-17","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-09-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}