{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T10:00:00Z","timestamp":1785146400016,"version":"3.55.0"},"reference-count":52,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,9,8]],"date-time":"2022-09-08T00:00:00Z","timestamp":1662595200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007398","name":"Strategiske Forskningsr\u00e5d","doi-asserted-by":"publisher","award":["Project Electromed 40 (11\u2010115313)"],"award-info":[{"award-number":["Project Electromed 40 (11\u2010115313)"]}],"id":[{"id":"10.13039\/100007398","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biomed Mater Res"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Pelvic organ prolapse (POP) affects many women, with an estimated lifetime risk of surgical intervention of 18.7%. There is a need for alternative approaches as the use of synthetic nondegradable mesh was stopped due to severe adverse events, and as current methods for pelvic floor repair have high POP recurrence rates. Thus, we hypothesized that electrospun degradable meshes with stem cells and growth factor were safe and durable for the long term in elderly rats. In an abdominal repair model, electrospun polycaprolactone (PCL) meshes coated with connective tissue growth factor (CTGF)\/PEG\u2010fibrinogen (PF) and rat mesenchymal stem cells were implanted in elderly female rats and removed after in average 53\u2009weeks (53\u2010week group). Collagen amount and production were quantified by qPCR and Western blotting. Moreover, histological appearance and biomechanical properties were evaluated. Results were compared with previous results of young rats with identical mesh implanted for 24\u2009weeks (24\u2010week group). The 53\u2010week group differed from the 24\u2010week group in terms of (1) reduced collagen III, (2) strong reduction in foreign body response, and (3) altered histological appearance. We found comparable biomechanical properties, aside from higher, not significant, mean tissue stiffness in the 53\u2010week group. Lastly, we identified mesh components 53\u2009weeks after implantation. This study provides new insights into future POP repair in postmenopausal women by showing how CTGF\/PF\u2010coated electrospun PCL meshes with stem cells exhibit sufficient support, biocompatibility, and no mesh\u2010related complications long term in an abdominal repair model in elderly rats.<\/jats:p>","DOI":"10.1002\/jbm.b.35158","type":"journal-article","created":{"date-parts":[[2022,9,8]],"date-time":"2022-09-08T21:44:42Z","timestamp":1662673482000},"page":"392-401","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Electrospun nanofiber mesh with connective tissue growth factor and mesenchymal stem cells for pelvic floor repair: Long\u2010term study"],"prefix":"10.1002","volume":"111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5805-1535","authenticated-orcid":false,"given":"Sofie Husted","family":"Laursen","sequence":"first","affiliation":[{"name":"Department of Gynaecology and Obstetrics Aarhus University Hospital  Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Signe Gellert","family":"Hansen","sequence":"additional","affiliation":[{"name":"Department of Medicine Soenderborg Hospital  Soenderborg Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehmet Berat","family":"Taskin","sequence":"additional","affiliation":[{"name":"Leibniz Institute of Polymer Research Dresden Max Bergmann Center of Biomaterials Dresden  Dresden Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Menglin","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Biological and Chemical Engineering \u2010 Medical Biotechnology Aarhus University  Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lise","family":"Wogensen","sequence":"additional","affiliation":[{"name":"Deans Office Health Aarhus University  Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jens Vinge","family":"Nygaard","sequence":"additional","affiliation":[{"name":"Department of Biological and Chemical Engineering \u2010 Medical Biotechnology Aarhus University  Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanne Maigaard","family":"Axelsen","sequence":"additional","affiliation":[{"name":"Department of Gynaecology and Obstetrics Aarhus University Hospital  Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,9,8]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00192-016-3003-y"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9378(99)70203-6"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.AOG.0000136100.10818.d8"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00192-014-2413-y"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00192-017-3395-3"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/SPV.0000000000000863"},{"key":"e_1_2_8_8_1","unstructured":"U.S. Food and Drug Administration.FDA takes action to protect women's health orders manufacturers of surgical mesh intended for transvaginal repair of pelvic organ prolapse to stop selling all devices. 2019. 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