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AMs undergo different processing with resultant changes in cellular content and structure. This study evaluates the effects of sidedness and processing on human corneal epithelial cell (HCEC) activity, the effect of processing on HCEC inflammatory response, and then a case study is presented. Three differently processed, commercially available ocular AMs were selected: (1) Biovance\u00ae3L Ocular, a decellularized, dehydrated human AM (DDHAM), (2) AMBIO2\u00ae, a dehydrated human AM (DHAM), and (3) AmnioGraft\u00ae, a cryopreserved human AM (CHAM). HCECs were seeded onto the AMs and incubated for 1, 4 and 7\u2009days. Cell adhesion and viability were evaluated using alamarBlue assay. HCEC migration was evaluated using a scratch wound assay. An inflammatory response was induced by TNF\u2010\u03b1 treatment. The effect of AM on the expression of pro\u2010inflammatory genes in HCECs was compared using quantitative polymerase chain reaction (qPCR). Staining confirmed complete decellularization and the absence of nuclei in DDHAM. HCEC activity was best supported on the stromal side of DDHAM. Under inflammatory stimulation, DDHAM promoted a higher initial inflammatory response with a declining trend across time. Clinically, DDHAM was used to successfully treat anterior basement membrane dystrophy. Compared with DHAM and CHAM, DDHAM had significant positive effects on the cellular activities of HCECs in vitro, which may suggest greater ocular cell compatibility in vivo.<\/jats:p>","DOI":"10.1002\/jbm.b.35186","type":"journal-article","created":{"date-parts":[[2022,11,12]],"date-time":"2022-11-12T17:24:55Z","timestamp":1668273895000},"page":"684-700","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["An in vitro comparison of human corneal epithelial cell activity and inflammatory response on differently designed ocular amniotic membranes and a clinical case study"],"prefix":"10.1002","volume":"111","author":[{"given":"Yong","family":"Mao","sequence":"first","affiliation":[{"name":"Department of Chemistry and Chemical Biology Rutgers University Laboratory for Biomaterials Research  Piscataway New Jersey USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole M.","family":"Protzman","sequence":"additional","affiliation":[{"name":"Department of Research Healthcare Analytics, LLC  Easton Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikita","family":"John","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Chemical Biology Rutgers University Laboratory for Biomaterials Research  Piscataway New Jersey USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam","family":"Kuehn","sequence":"additional","affiliation":[{"name":"Celularity Inc.  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