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Two different naturally sourced membranes were evaluated in this study: (i) an intact fish skin membrane (Kerecis Oral\u00ae, Kerecis) and (ii) a porcine derived collagen (Mucograft\u00ae, Geistlich) membrane, positive control. For the <jats:italic>in vitro<\/jats:italic> experiments, human osteoprogenitor (hOP) cells were used to assess the cellular viability and proliferation at 24, 48, 72, and 168\u2009h. ALPL, COL1A1, BMP2, and RUNX2 expression levels were analyzed by real\u2010time PCR at 7 and 14\u2009days. The preclinical component was designed to mimic a GBR model in canines (<jats:italic>n<\/jats:italic>\u2009=\u200912). The first step was the extraction of premolars (P1\u2013P4) and the 1st molars bilaterally, thereby creating four three\u2010wall box type defects per mandible (two per side). Each defect site was filled with bone grafting material, which was then covered with one of the two membranes (Kerecis Oral\u00ae or Mucograft\u00ae). The groups were nested within the mandibles of each subject and membranes randomly allocated among the defects to minimize potential site bias. Samples were harvested at 30\u2010, 60\u2010, and 90\u2010days and subjected to computerized microtomography (\u03bcCT) for three\u2010dimensional reconstruction to quantify bone formation and graft degradation, in addition to histological processing to qualitatively analyze bone regeneration. Neither the intact fish skin membrane nor porcine\u2010based collagen membrane presented cytotoxic effects. An increase in cell proliferation rate was observed for both membranes, with the Kerecis Oral\u00ae outperforming the Mucograft\u00ae at the 48\u2010 and 168\u2010hour time points. Kerecis Oral\u00ae yielded higher ALPL expression relative to Mucograft\u00ae at both 7\u2010 and 14\u2010day points. Additionally, higher COL1A1 expression was observed for the Kerecis Oral\u00ae membrane after 7\u2009days but no differences were detected at 14\u2009days. The membranes yielded similar BMP2 and RUNX2 expression at 7 and 14\u2009days. Volumetric reconstructions and histologic micrographs indicated gradual bone ingrowth along with the presence of particulate bone grafts bridging the defect walls for both Kerecis Oral\u00ae and Mucograft\u00ae membranes, which allowed for the reestablishment of the mandible shape after 90\u2009days. New bone formation significantly increased from 30 to 60\u2009days, and from 60 to 90\u2009days <jats:italic>in vivo<\/jats:italic>, without significant differences between membranes. The amount of bovine grafting material (%) within the defects significantly decreased from 30 to 90\u2009days. Collagen membranes led to an upregulation of cellular proliferation and adhesion along with increased expression of genes associated with bone healing, particularly the intact fish skin membrane. Despite an increase in the bone formation rate in the defect over time, there was no significant difference between the membranes.<\/jats:p>","DOI":"10.1002\/jbm.b.35299","type":"journal-article","created":{"date-parts":[[2023,7,20]],"date-time":"2023-07-20T09:32:30Z","timestamp":1689845550000},"page":"1966-1978","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Osteogenic differentiation and reconstruction of mandible defects using a novel resorbable membrane: An in vitro and in vivo experimental study"],"prefix":"10.1002","volume":"111","author":[{"given":"Edmara T. 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