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Acellular porcine cornea (APC) is a promising corneal substitute to alleviate the shortage of corneal resources. The residual DNA was significantly reduced to 18.50\u2009\u00b1\u20093.19\u2009ng\/mg, and the clearance rate of \u03b1\u2010Gal was close to 100% after the treatment with freezing\u2013thawing combined enzyme, indicating that the decellularization was effective. The effects of different E\u2010beam doses at 0, 2, 8, 15, and 25\u2009kGy on the APC were studied. With the increase in irradiation dose, the transmittance, tensile strength, and swelling ratio of APC gradually decreased, but the resistance to enzymatic degradation was stronger than that of non\u2010irradiated APC, especially at 8\u2009kGy. The structure of APC was denser after irradiation, but the dose of 25\u2009kGy could cause partial collagen fiber fracture and increase the pore size. The cell viability of the APC irradiated by 15 and 25\u2009kGy were greater than 80%. After the implantation in rabbit corneas, there was no obvious neovascularization and inflammation, but the dose of 25\u2009kGy had a more destructive effect on the chemical bonds of collagen, which made the APC easier to be degraded. The thickness of APC in the 25\u2009kGy group was thinner than that in the 15\u2009kGy group 1\u2009year after surgery, and the epithelium grew more slowly, so the E\u2010beam dose of 15\u2009kGy might be more suitable for the sterilization of APC.<\/jats:p>","DOI":"10.1002\/jbm.b.35301","type":"journal-article","created":{"date-parts":[[2023,7,21]],"date-time":"2023-07-21T10:43:19Z","timestamp":1689936199000},"page":"2013-2024","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Structure and properties of the acellular porcine cornea irradiated with electron beam and its in\u2010situ implantation"],"prefix":"10.1002","volume":"111","author":[{"given":"Qing","family":"Li","sequence":"first","affiliation":[{"name":"Key Laboratory for Liquid\u2010Solid Structural Evolution and Processing of Materials (Ministry of Education), School of Materials Science and Engineering Shandong University  Jinan Shandong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yajun","family":"Sun","sequence":"additional","affiliation":[{"name":"Research and Development Center Qingdao Chunghao Tissue Engineering Co., Ltd.  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