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Oxidative stress and inflammation owing to lifestyle changes may lead to serious diseases such as Cancers, Gout, and Arthritis etc. These disorders can be prevented using different therapeutic strategies including nanomedicine. Biosynthesized gold nanoparticles (GNPs) because of their anti\u2010inflammatory and antioxidant bioactivities can be key player in reversal of these ailments. This study was carried out to evaluate the anti\u2010inflammatory and antioxidant potential of bio fabricated GNPs with <jats:italic>Sarcococca saligna<\/jats:italic> (<jats:italic>S. saligna<\/jats:italic>) extract on injured human adipose\u2010derived Mesenchymal stem cells (hADMSCs). GNPs were characterized by ultraviolet\u2013visible (UV\u2013Vis) spectroscopy, Scanning Electron Microscopy (SEM), x\u2010ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and energy dispersive x\u2010ray (EDS). Phytochemical screening of biosynthesized GNPs exhibited a significant release of polyphenols, that is, total phenolic content (TPC) and total flavonoid content (TFC). GNPs priming amended the in vitro injury caused by Monosodium Iodoacetate (MIA) as exhibited by improved cell viability, wound closure response and superoxide dismutase activity (SOD). The anti\u2010inflammatory conduct assessed through NF\u2010\u03baB pathway and other associated inflammatory markers reported down\u2010regulation of TNF\u2010\u03b1 (0.644\u2009\u00b1\u20090.045), IL\u20101\u03b2 (0.694\u2009\u00b1\u20090.147) and IL\u20106 (0.622\u2009\u00b1\u20090.112), apoptosis causing genes like Caspase\u20103 (0.734\u2009\u00b1\u20090.13) and BAX (0.830\u2009\u00b1\u20090.12), NF\u2010\u03baB pathway, p65 (0.672\u2009\u00b1\u20090.084) and p105 (0.539\u2009\u00b1\u20090.083) associated genes. High SOD activity (95\u2009\u00b1\u20095.25%) revealed by treated hADMSCs with GNPs also supported the antioxidant role of GNPs <jats:italic>in vitro<\/jats:italic> model. This study concludes that <jats:italic>S. saligna<\/jats:italic> bio fabricated GNPs priming may improve the therapeutic potential of hADMSCs against chronic inflammatory problems by regulating NF\u2010\u03baB pathway.<\/jats:p>","DOI":"10.1002\/jbm.b.35303","type":"journal-article","created":{"date-parts":[[2023,8,10]],"date-time":"2023-08-10T12:52:53Z","timestamp":1691671973000},"page":"2032-2043","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["<i>Sarcococca saligna<\/i> fabricated gold nanoparticles alleviated <i>in vitro<\/i> oxidative stress and inflammation in human adipose\u2010derived stem cells"],"prefix":"10.1002","volume":"111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1878-1610","authenticated-orcid":false,"given":"Nadia","family":"Naseer","sequence":"first","affiliation":[{"name":"Center of Excellence in Molecular Biology (CEMB) University of the Punjab  Lahore 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