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Glass ceramics produced by different synthesis routes may result in changes in the material physicochemical properties and consequently affect the success or failure of the bone healing response. To investigate the differences in the orchestration of the inflammatory and healing process in bone grafting and repair using different glass\u2013ceramic routes production. Thirty male Wistar rats underwent surgical unilateral parietal defects filled with silicate glass\u2013ceramic produced by distinct routes: BS \u2013 particulate glass\u2013ceramic produced via the fusion\/solidification route, and BG \u2013 particulate glass\u2013ceramic produced via the sol\u2013gel route. After 7, 14, and 21\u2009days from biomaterial grafting, parietal bones were removed to be analyzed under H&amp;E and Massons' Trichome staining, and immunohistochemistry for CD206, iNOS, and TGF\u2010\u03b2. Our findings demonstrated that the density of lymphocytes and plasma cells was significantly higher in the BS group at 45, and 7\u2009days compared to the BG group, respectively. Furthermore, a significant increase of foreign body giant cells (FBGCs) in the BG group at day 7, compared to BS was found, demonstrating early efficient recruitment of FBGCs against sol\u2013gel\u2010derived glass\u2013ceramic particulate (BS group). According to macrophage profiles, CD206<jats:sup>+<\/jats:sup> macrophages enhanced at the final periods of both groups, being significantly higher at 45\u2009days of BS compared to the BG group. On the other hand, the density of transformation growth factor beta (TGF\u2010\u03b2) positive cells on 21\u2009days were the highest in BG, and the lowest in the BS group, demonstrating a differential synergy among groups. Noteworthy, TGF\u2010\u03b2<jats:sup>+<\/jats:sup> cells were significantly higher at 21\u2009days of BG compared to the BS group. Glass\u2013ceramic biomaterials can act differently in the biological process of bone remodeling due to their route production, being the sol\u2013gel route more efficient to activate M2 macrophages and specific FBGCs compared to the traditional route. Altogether, these features lead to a better understanding of the effectiveness of inflammatory response for biomaterial degradation and provide new insights for further preclinical and clinical studies involved in bone healing.<\/jats:p>","DOI":"10.1002\/jbm.b.35340","type":"journal-article","created":{"date-parts":[[2023,11,6]],"date-time":"2023-11-06T06:45:30Z","timestamp":1699253130000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Effects of glass\u2013ceramic produced by the sol\u2013gel route in macrophages recruitment and polarization into bone tissue regeneration"],"prefix":"10.1002","volume":"112","author":[{"given":"Raquel Barroso Parra","family":"da Silva","sequence":"first","affiliation":[{"name":"Department of Basic Sciences S\u00e3o Paulo State University (Unesp), School of Dentistry  Ara\u00e7atuba Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia 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