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However, there are few explorations using biodegradable Mg for cardiovascular stent application. In this present study, sirolimus\u2010loaded poly D, L\u2010lactic\u2010co\u2010glycolic acid (PLGA)\u2010coated\/ sirolimus\u2010fixed\/AZ91 Mg alloy\u2010based substrate was developed via a layer\u2010by\u2010layer approach for cardiovascular stent application. The AZ91 Mg alloy was prepared through the squeeze casting technique. The casted AZ91 Mg alloy (Mg) was alkali\u2010treated to provide macroporous networks to hold the sirolimus and PLGA layers. The systematic characterization was investigated via electrochemical, optical, physicochemical, and in\u2010vitro biological characteristics. The presence of the Mg<jats:sub>17<\/jats:sub>Al<jats:sub>12<\/jats:sub> phase in the Mg sample was found in the x\u2010ray diffraction system (XRD) spectrum which influences the corrosion behavior of the developed substrate. The alkali treatment increases the substrate's hydrophilicity which was confirmed through static contact angle measurement. The anti\u2010corrosion characteristic of casted\u2010AZ91 Mg alloy (Mg) was slightly less than the sirolimus\u2010loaded PLGA\u2010coated alkali\u2010treated AZ91 Mg alloy (Mg\/Na\/S\/P) substrate. However, dissolution rates for both substrates were found to be controlled at cell culture conditions. Radiographic densities of AZ91 Mg alloy substrates (Mg, Mg\/Na, and Mg\/Na\/S\/P) were measured to be 0.795\u2009\u00b1\u20090.015, 0.742\u2009\u00b1\u20090.01, and 0.712\u2009\u00b1\u20090.017, respectively. The star\u2010shaped structure of 12% sirolimus\/PLGA ensures the bioavailability of the drugs. Sirolimus release kinetic was fitted up to 80% with the \u201cHiguchi model\u201d for Mg samples, whereas Mg\/Na\/S\/P showed 45% fitting with a zero\u2010order mechanism. The Mg\/Na\/S\/P substrate showed a 70% antithrombotic effect compared to control<jats:italic>.<\/jats:italic> Further, alkali treatment enhances the antibacterial characteristic of AZ91 Mg alloy. Also, the alkali\u2010treated sirolimus\u2010loaded substrates (Mg\/Na\/S and Mg\/Na\/S\/P) inhibit the valvular interstitial cell's growth significantly in in\u2010vitro. Hence, the results imply that sirolimus\u2010loaded PLGA\u2010coated AZ91 Mg alloy\u2010based substrate can be a potential candidate for cardiovascular stent application.<\/jats:p>","DOI":"10.1002\/jbm.b.35350","type":"journal-article","created":{"date-parts":[[2023,11,15]],"date-time":"2023-11-15T16:37:24Z","timestamp":1700066244000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Biodegradable <scp>AZ91<\/scp> magnesium alloy\/sirolimus\/poly D, L\u2010lactic\u2010co\u2010glycolic acid\u2010based substrate for cardiovascular device application"],"prefix":"10.1002","volume":"112","author":[{"given":"Monalisha","family":"Mohanta","sequence":"first","affiliation":[{"name":"Department of Biotechnology and Medical Engineering National Institute of Technology Rourkela  Rourkela Odisha 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