{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T09:55:31Z","timestamp":1753869331666,"version":"3.41.2"},"reference-count":50,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,3,23]],"date-time":"2024-03-23T00:00:00Z","timestamp":1711152000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100023581","name":"National Science Foundation Graduate Research Fellowship Program","doi-asserted-by":"publisher","award":["DGE\u20101842166"],"award-info":[{"award-number":["DGE\u20101842166"]}],"id":[{"id":"10.13039\/100023581","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biomed Mater Res"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Hemorrhage is the second leading cause of death in patients under 46\u2009years of age in the United States. Cessation of hemorrhage prevents hemorrhagic shock and tissue hypoxia. Controlling the bleed via direct pressure or tourniquet is often the first line of defense, but long\u2010term care requires staples, hemostatic agents, or sealants that seal the vessel and restore blood flow. Here, we compare a new photocurable extracellular matrix sealant (pcECM) with low, medium, and high crosslink density formulations to a commercially available fibrin\u2010based sealant, TISSEEL\u00ae. pcECM has potential uses in surgical and remote settings due to room temperature storage conditions and fast preparation time. Here, we determine if pcECM sealant can stop venous hemorrhage in a murine model, adhere to the wound site <jats:italic>in vivo<\/jats:italic> throughout the wound\u2010healing process, and has the mechanical properties necessary for stopping hemorrhage. Adjusting pcECM crosslinking density significantly affected viscosity, swelling, burst strength, tensile strength, and elasticity of the sealant. 3\u2010Dimensional ultrasound volume segmentations showed pcECM degrades to 17\u2009\u00b1\u20098% of its initial implant volume by day 28. Initially, local hemodynamic changes were observed, but returned close to baseline levels by day 28. Acute inflammation was observed near the puncture site in pcECM implanted mice, and we observed inflammatory markers at the 14\u2010day explant for both sealants. pcECM and fibrin sealant successfully sealed the vessel in all cases, and consistently degraded over 14\u201328\u2009days. pcECM is a durable sealant with tunable mechanical properties and possible uses in hemorrhage control and other surgical procedures.<\/jats:p>","DOI":"10.1002\/jbm.b.35401","type":"journal-article","created":{"date-parts":[[2024,3,23]],"date-time":"2024-03-23T18:15:11Z","timestamp":1711217711000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Photocurable extracellular matrix sealant for cessation of venous hemorrhage"],"prefix":"10.1002","volume":"112","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3534-9440","authenticated-orcid":false,"given":"Luke E.","family":"Schepers","sequence":"first","affiliation":[{"name":"Weldon School of Biomedical Engineering Purdue University  West Lafayette Indiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brooke L.","family":"Martindale","sequence":"additional","affiliation":[{"name":"Product Engineering Cook Biotech Inc.  West Lafayette Indiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alycia G.","family":"Berman","sequence":"additional","affiliation":[{"name":"Product Engineering Cook Biotech Inc.  West Lafayette Indiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hannah L.","family":"Cebull","sequence":"additional","affiliation":[{"name":"Weldon School of Biomedical Engineering Purdue University  West Lafayette Indiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William","family":"Van Alstine","sequence":"additional","affiliation":[{"name":"Pathology Cook Research Inc.  West Lafayette Indiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sydney E.","family":"Hollingshead","sequence":"additional","affiliation":[{"name":"Product Engineering Cook Biotech Inc.  West Lafayette Indiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tyler","family":"Novak","sequence":"additional","affiliation":[{"name":"Product Engineering Cook Biotech Inc.  West Lafayette Indiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8883-7953","authenticated-orcid":false,"given":"Craig J.","family":"Goergen","sequence":"additional","affiliation":[{"name":"Weldon School of Biomedical Engineering Purdue University  West Lafayette Indiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,3,23]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/milmed\/usy185"},{"volume-title":"StatPearls","year":"2022","author":"Donley ER","key":"e_1_2_9_3_1"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/cc2407"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.wem.2016.12.005"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.b.33409"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.35052"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1517\/14712590802689258"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2018.04.034"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1177\/0885328211426491"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2010.07.032"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2008.12.059"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.32572"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1517\/14712598.2013.848193"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365\u20102516.1998.440449.x"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1039\/9781788012720\u201000210"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.21873\/invivo.11820"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.spinee.2010.09.017"},{"key":"e_1_2_9_19_1","first-page":"414","article-title":"Use and efficacy of hemostats in neurosurgery","volume":"37","author":"Signorelli F","year":"2020","journal-title":"Surg Technol Int"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1097\/PRS.0b013e3182131cf5"},{"issue":"5","key":"e_1_2_9_21_1","first-page":"1102","article-title":"Use of Tisseel fibrin sealant in neurosurgical procedures: incidence of cerebrospinal fluid leaks and cost\u2010benefit analysis in a retrospective study","volume":"52","author":"Kassam A","year":"2003","journal-title":"Neurosurgery"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1177\/014556130408300413"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joms.2016.04.027"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0003\u20104975(10)62298\u20109"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600\u2010051x.1992.tb01155.x"},{"key":"e_1_2_9_26_1","unstructured":"TISSEEL.Baxter specialty Site. Accessed April 12 2022.https:\/\/advancedsurgery.baxter.com\/tisseel"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11605\u2010011\u20101771\u20108"},{"issue":"6","key":"e_1_2_9_28_1","first-page":"756","article-title":"Comparison of traditional and integrated digital anesthetic vaporizers","volume":"54","author":"Damen FW","year":"2015","journal-title":"J Am Assoc Lab Anim Sci"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.3791\/61311"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph10020036"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/phar.1291"},{"issue":"3","key":"e_1_2_9_32_1","first-page":"323","article-title":"Evaluation of the topical hemostatic efficacy and safety of TISSEEL VH S\/D fibrin sealant compared with currently licensed TISSEEL VH in patients undergoing cardiac surgery: a phase 3, randomized, double\u2010blind clinical study","volume":"48","author":"Lowe J","year":"2007","journal-title":"J Cardiovasc Surg (Torino)"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/btm2.10240"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mtbio.2021.100199"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0003\u20104975(01)02424\u20109"},{"issue":"1","key":"e_1_2_9_36_1","first-page":"P6","article-title":"BioGlue in 2011: what is its role in cardiac surgery?","volume":"44","author":"Bhamidipati CM","year":"2012","journal-title":"J Extra Corpor Technol"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1532\/hsf98.20061066"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijscr.2016.04.010"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.12998\/wjcc.v9.i20.5668"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2021.112208"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.3389\/fchem.2021.744099"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.201200316"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0939\u20106411(00)00090\u20104"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1152\/advan.00141.2019"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1152\/advan.00182.2018"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.3.5663.148"},{"volume-title":"Guyton and Hall Textbook of Medical Physiology","year":"2020","author":"Guyton AC, Hall JE, Hall ME.","key":"e_1_2_9_47_1"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.3238\/arztebl.2018.0557"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejpb.2015.05.022"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2544"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1242\/dmm.004077"}],"container-title":["Journal of Biomedical Materials Research Part B: Applied Biomaterials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbm.b.35401","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,4,15]],"date-time":"2024-04-15T14:09:58Z","timestamp":1713190198000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbm.b.35401"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,3,23]]},"references-count":50,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2024,4]]}},"alternative-id":["10.1002\/jbm.b.35401"],"URL":"https:\/\/doi.org\/10.1002\/jbm.b.35401","archive":["Portico"],"relation":{},"ISSN":["1552-4973","1552-4981"],"issn-type":[{"type":"print","value":"1552-4973"},{"type":"electronic","value":"1552-4981"}],"subject":[],"published":{"date-parts":[[2024,3,23]]},"assertion":[{"value":"2023-08-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-02-18","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-03-23","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e35401"}}