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After flattening the enamel surface with 600, 1200, and 2000 grit sandpaper, the samples were divided into two groups based on the challenges they underwent: cariogenic (0.1\u2009M lactic acid at pH\u20095.0) or erosive (0.05\u2009M citric acid solution at pH\u20092.3). Samples from each challenge were further categorized into 11 groups (<jats:italic>n<\/jats:italic>\u2009=\u20099) according to the duration of cariogenic (3, 7, and 14\u2009days) or erosive (3, 7, and 10\u2009days) challenge, along with positive control groups (fragments untreated with challenges and treated with different Biosilicate concentrations) and negative controls (fragments treated with artificial saliva for the same periods established for cariogenic and erosive challenges). Treatments with orodispersible films containing Biosilicate (30 and 50\u2009mg) were administered for 2\u2009min per day for 15\u2009days.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The highest remineralizing potential was observed in samples treated with Biosilicate after 14\u2009days of cariogenic challenge, irrespective of the concentration tested. For samples subjected to erosive challenge, erosion time did not affect Biosilicate's remineralizing potential.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Biosilicate shows promise in terms of remineralizing potential in enamel subjected to cariogenic challenge due to its ability to form hydroxycarbonapatite in mineralized tissues.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jbm.b.35446","type":"journal-article","created":{"date-parts":[[2024,6,27]],"date-time":"2024-06-27T07:15:47Z","timestamp":1719472547000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Development and effect of orodispersible film incorporated with Biosilicate for remineralization of dental enamel subjected to cariogenic and erosive challenge"],"prefix":"10.1002","volume":"112","author":[{"given":"Ayodele Alves","family":"Amorim","sequence":"first","affiliation":[{"name":"Department of Dental Materials and Prosthodontics, Ribeir\u00e3o Preto School of Dentistry University of S\u00e3o Paulo  Ribeir\u00e3o Preto Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo Jos\u00e9","family":"Soares","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry, Ribeir\u00e3o Preto School of Dentistry University of S\u00e3o Paulo  Ribeir\u00e3o Preto Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1455-1538","authenticated-orcid":false,"given":"Fernanda de Carvalho Panzeri","family":"Pires\u2010de\u2010Souza","sequence":"additional","affiliation":[{"name":"Department of Dental Materials and Prosthodontics, Ribeir\u00e3o Preto School of Dentistry University of S\u00e3o Paulo  Ribeir\u00e3o Preto Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,6,27]]},"reference":[{"issue":"3","key":"e_1_2_10_2_1","first-page":"131","article-title":"Influence of five home whitening gels and a remineralizing gel on the enamel and dentin ultrastructure and hardness","volume":"24","author":"Pinheiro H","year":"2011","journal-title":"Am J Dent"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sjdr.2016.10.001"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0182406"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/26415275.2021.1884558"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.5005\/jp-journals-10024-2277"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dental.2019.05.021"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.17219\/dmp\/146038"},{"issue":"6","key":"e_1_2_10_9_1","first-page":"ZC39","article-title":"An in vitro comparative evaluation of three remineralizing agents using confocal microscopy","volume":"10","author":"Chokshi K","year":"2016","journal-title":"J Clin Diagn Res"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/ma11091636"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1590\/1678-7757-2021-0120"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdent.2014.05.006"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1590\/0103-6440202304904"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdent.2021.103688"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdent.2020.103402"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.22607"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dental.2019.05.009"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dental.2022.06.016"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00784-022-04796-0"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0143957"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1590\/0103-6440201601524"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1590\/0103-6440202305636"},{"issue":"6","key":"e_1_2_10_23_1","first-page":"619","article-title":"Remineralization of demineralized dentin induced by bioactive glass NovaMin. 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