{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T20:57:05Z","timestamp":1780952225499,"version":"3.54.1"},"reference-count":41,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2024,11,7]],"date-time":"2024-11-07T00:00:00Z","timestamp":1730937600000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biomed Mater Res"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The emergence of hybrid scaffolds, blending biomaterials with diverse properties, offers promise in musculoskeletal tissue engineering. However, a need for in\u00a0vitro platforms investigating biological behavior and the interplay of different load\u2010bearing and colonizable synthetic bone substitute materials remains. Herein, we present a novel, in\u2010house producible, and scalable clamp culture system designed for facile in\u00a0vitro analysis of interactions between biomaterials, hydrogels, and cells. The system, constructed here from an exemplary 3D\u2010printable polymer and photopolymerizable hydrogel using a widely available benchtop 3D printer, ensures mechanical stability and protection for the embedded hydrogel via its double\u2010clamp structure, facilitating various analytical methods while preserving culture integrity. Hybrid clamp cultures were additively manufactured from polylactic acid, filled with a bone precursor cell\u2010laden methacrylate gelatin hydrogel, cultured for 14\u2009days, and analyzed for cell viability, mineralization, and osseous differentiation. Results indicate no adverse effects on osteogenic differentiation or mineralization compared to conventional droplet cultures, with enhanced cell viability and simplified handling and downstream analysis. This system demonstrates the potential for robust experimentation in tissue engineering and is adaptable to various plate formats, and thus highly suitable for the investigation of biomaterial\u2010cell interactions and the development of implants for musculoskeletal tissue defects.<\/jats:p>","DOI":"10.1002\/jbm.b.35494","type":"journal-article","created":{"date-parts":[[2024,11,7]],"date-time":"2024-11-07T14:04:56Z","timestamp":1730988296000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Additively Manufactured <scp>3D<\/scp> Clamp\u2010Culture System for the Investigation of Material\u2010Cell Interactions in Multi\u2010Material Hybrid Scaffolds for Musculoskeletal Tissue Defects"],"prefix":"10.1002","volume":"112","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3003-591X","authenticated-orcid":false,"given":"Simon","family":"Enbergs","sequence":"first","affiliation":[{"name":"Charit\u00e9 \u2013 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