{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T01:37:00Z","timestamp":1768268220254,"version":"3.49.0"},"reference-count":34,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T00:00:00Z","timestamp":1770249600000},"content-version":"am","delay-in-days":369,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#am"},{"start":{"date-parts":[[2025,2,5]],"date-time":"2025-02-05T00:00:00Z","timestamp":1738713600000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100007472","name":"University of Kentucky","doi-asserted-by":"publisher","award":["NIH\/NIGMS#P20GM148326"],"award-info":[{"award-number":["NIH\/NIGMS#P20GM148326"]}],"id":[{"id":"10.13039\/100007472","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000005","name":"U.S. Department of Defense","doi-asserted-by":"publisher","award":["CDMRP\/SCIRP#W81XWH2010347"],"award-info":[{"award-number":["CDMRP\/SCIRP#W81XWH2010347"]}],"id":[{"id":"10.13039\/100000005","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["NIHR01#NS119337"],"award-info":[{"award-number":["NIHR01#NS119337"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biomed Mater Res"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Traumatic spinal cord injury (SCI) presents a significant medical challenge due to its intricate nature and treatment complexities. SCI can cause physical impairments by affecting neural and motor functions as well as initiating a series of pathophysiological events exacerbating the initial trauma. Leakage from ruptured neurons and vessels disrupt ionic balance and induces excitotoxicity, leading to progressive cellular degeneration. Introducing mitochondria to the SCI lesion has shown potential in attenuating secondary injury. Mitochondria transplantation improves cellular bioenergetics and reduces concentration of reactive oxygen species achieving homeostasis and neuroprotection. Nonetheless, keeping mitochondria viable outside cell environment for a time longer than a few minutes proves to be challenging. Additionally, localized delivery to the injury site has also been limited by other factors including flow rate of cerebrospinal fluid that washes away mobilized organelle from the compromised tissue site. Previously we showed that using hyaluronic acid\u2010methylcellulose semi\u2010gels (HAMC) as a biocompatible, erodible thermogelling delivery vehicle helped to overcome some of these challenges. HAMC allows for controlled release at and around the injury site, utilizing the reverse thermogelling property of MC. Sustained release of mitochondria at slower rate can increase their uptake in spinal tissue. To better optimize the semi\u2010gel delivery of mitochondria requires a more complete understanding of the physicochemical properties of the HAMC semi\u2010gels. We have used ultraviolet\u2013visible spectroscopy to measure optical density of HAMC semi\u2010gels for different HA to MC ratios and examine the temperature dependent gelation properties above their low critical solution temperature (LCST). The viscosity and degree of crystallinity of the resulting HAMC semi\u2010gels were also assessed. Semi\u2010gel erosion and mitochondrial release over time were studied using a fluorescence microplate reader. Lastly, seahorse assay was used to study released mitochondria respiration and viability after incubation in HAMC semi\u2010gel.<\/jats:p>","DOI":"10.1002\/jbm.b.35537","type":"journal-article","created":{"date-parts":[[2025,2,5]],"date-time":"2025-02-05T06:21:32Z","timestamp":1738736492000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Physicochemical Characterization of Hyaluronic Acid\u2010Methylcellulose Semi\u2010Gels for Mitochondria Transplantation"],"prefix":"10.1002","volume":"113","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6194-2144","authenticated-orcid":false,"given":"A. Jamie","family":"Ahmed","sequence":"first","affiliation":[{"name":"Department of Chemical and Materials Engineering, Stanley and Karen Pigman College of Engineering University of Kentucky  Lexington Kentucky USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zoe A.","family":"Gallegos","sequence":"additional","affiliation":[{"name":"Department of Chemistry, College of Arts and Sciences University of Kentucky  Lexington Kentucky USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Md Abu Monsur","family":"Dinar","sequence":"additional","affiliation":[{"name":"Department of Chemistry, College of Arts and Sciences University of Kentucky  Lexington Kentucky USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick G.","family":"Sullivan","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, College of Medicine University of Kentucky  Lexington Kentucky USA"},{"name":"Lexington VA Healthcare System  Lexington Kentucky USA"},{"name":"Spinal Cord &amp; Brain Injury Research Center University of Kentucky  Lexington Kentucky USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jason E.","family":"DeRouchey","sequence":"additional","affiliation":[{"name":"Department of Chemistry, College of Arts and Sciences University of Kentucky  Lexington Kentucky USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samir P.","family":"Patel","sequence":"additional","affiliation":[{"name":"Spinal Cord &amp; Brain Injury Research Center University of Kentucky  Lexington Kentucky USA"},{"name":"Department of Physiology, College of Medicine University of Kentucky  Lexington Kentucky USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander G.","family":"Rabchevsky","sequence":"additional","affiliation":[{"name":"Spinal Cord &amp; Brain Injury Research Center University of Kentucky  Lexington Kentucky USA"},{"name":"Department of Physiology, College of Medicine University of Kentucky  Lexington Kentucky USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas D.","family":"Dziubla","sequence":"additional","affiliation":[{"name":"Department of Chemical and Materials Engineering, Stanley and Karen Pigman College of Engineering University of Kentucky  Lexington Kentucky USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,2,5]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1310\/sci1604\u20101"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.5312\/wjo.v6.i1.8"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2017.18"},{"key":"e_1_2_10_5_1","unstructured":"National Spinal Cord Injury Statistical Center \u201cSpinal Cord Injury Facts and Figures at a Glance; Birmingham AL 2021.\u201dwww.msktc.org\/sci\/model\u2010system\u2010centers."},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fneur.2019.00282"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2020.113332"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/7610414"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.MITO.2022.04.002"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1089\/NEU.2017.5605"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2022.931800"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsps.2019.08.001"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/ACS.BIOMAC.8B00392"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2019.01.001"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2014.06.020"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/CELLS9092113"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.engreg.2020.10.001"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41385\u2010021\u201000409\u20103"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40824\u2010022\u201000310\u20105"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s42114\u2010023\u201000665\u2010w"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainresbull.2019.03.005"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2018.11.012"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2014.04.026"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.macromol.9b01773"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/polb.24065"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/app.39145"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/polym9030091"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.3390\/gels7040205"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.3390\/polym14091810"},{"key":"e_1_2_10_30_1","unstructured":"P. W.Schmidt T. P.Lodge andF. S.Bates \u201cFibrillar Structure and Networks in Methylcellulose Hydrogels; 2018.\u201d"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/S41598\u2010020\u201058417\u20105"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010021\u201082884\u2010z"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmbbm.2017.09.031"},{"key":"e_1_2_10_34_1","unstructured":"L. K.Gilmer K. N.Roberts K.Joy P. G.Sullivan andS. W.Scheff \u201cEarly Mitochondrial Dysfunction After Cortical Contusion Injury.\u201d"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2020.00153"}],"container-title":["Journal of Biomedical Materials Research Part B: Applied Biomaterials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/am-pdf\/10.1002\/jbm.b.35537","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbm.b.35537","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,23]],"date-time":"2025-07-23T07:03:59Z","timestamp":1753254239000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbm.b.35537"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2]]},"references-count":34,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2025,2]]}},"alternative-id":["10.1002\/jbm.b.35537"],"URL":"https:\/\/doi.org\/10.1002\/jbm.b.35537","archive":["Portico"],"relation":{},"ISSN":["1552-4973","1552-4981"],"issn-type":[{"value":"1552-4973","type":"print"},{"value":"1552-4981","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2]]},"assertion":[{"value":"2024-04-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-06","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e35537"}}