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However, free colchicine demonstrated cytotoxicity to ocular cells and cannot directly be used for eye disease. Thus, this study introduces, for the first time, the development and use of colchicine\u2010encapsulated liposomes as a novel therapeutic approach for managing inflammation\u2010driven ocular conditions. The encapsulation of colchicine within liposomes represents a significant innovation, aimed at enhancing biocompatibility and therapeutic efficacy while minimizing cytotoxic effects associated with free colchicine. Our research synthesized colchicine\u2010loaded liposomes and assessed their therapeutic impact on human monocytes, macrophages, and retinal pigment epithelium (RPE) cells in an inflammatory environment. The findings reveal a groundbreaking improvement in treatment strategies, with a substantial reduction in TNF\u2010alpha\u2010induced reactive oxygen species (ROS) and nitric oxide (NO) production in RPE cells. Moreover, the colchicine\u2010loaded liposomes significantly inhibited the proliferation and ROS production in activated monocytes and macrophages and effectively decreased interleukin (IL)\u20101\u03b2 and IL\u20106 secretion, highlighting their strong anti\u2010inflammatory properties and showed slightly better suppression of these two cytokines than dexamethasone\u2010liposomes.<\/jats:p>","DOI":"10.1002\/jbm.b.35540","type":"journal-article","created":{"date-parts":[[2025,1,31]],"date-time":"2025-01-31T21:04:21Z","timestamp":1738357461000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Biosafety and Efficacy Studies of Colchicine\u2010Encapsulated Liposomes for Ocular Inflammatory Diseases"],"prefix":"10.1002","volume":"113","author":[{"given":"Lu","family":"Yuan","sequence":"first","affiliation":[{"name":"Department of Ophthalmology Children's Hospital, Zhejiang University School of Medicine, National 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