{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T06:13:34Z","timestamp":1789712014737,"version":"4.0.1"},"reference-count":41,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,1,31]],"date-time":"2025-01-31T00:00:00Z","timestamp":1738281600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004410","name":"T\u00fcrkiye Bilimsel ve Teknolojik Ara\u015ft\u0131rma Kurumu","doi-asserted-by":"publisher","award":["120Z942"],"award-info":[{"award-number":["120Z942"]}],"id":[{"id":"10.13039\/501100004410","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018847","name":"Sel\u00e7uk University Research Foundation","doi-asserted-by":"publisher","award":["21406003"],"award-info":[{"award-number":["21406003"]}],"id":[{"id":"10.13039\/501100018847","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biomed Mater Res"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Wounds, disruptions in normal anatomy, are classified as acute or chronic. The choice of wound treatment relies significantly on dressing materials. Electrospun nanofibrous materials offer promising applications in wound healing, featuring a substantial surface area, close mimicry of the natural extracellular matrix, and adjustable water resistance, air permeability, and drug release. This research endeavors to formulate an innovative three\u2010layered nanofibrous wound dressing using the electrospinning technique with the primary objectives of enhancing patient well\u2010being, exhibiting antimicrobial characteristics, and expediting wound healing. The designed dressing comprises nanofibers of polyurethane (PU), quercetin (Q)\u2010loaded polyethylene glycol (PEG), polyvinyl alcohol (PVA), and gelatin. Characterization of individual layers and the integrated wound dressing was conducted through SEM and FT\u2010IR analyses. The efficacy of the nanofibrous wound dressing was assessed through in\u00a0vitro human cell culture and in\u00a0vivo rat wound models. The anti\u2010toxic effects of nanofiber wound dressing on human epithelial and keratin cells have been proven. In\u00a0vitro wound models in 24\u2010well plates were utilized to assess the impact on wound healing rates. Photographic documentation of wound closure was performed at the different treatment hours, revealing complete closure of the wounds by the end of the 48th hour. Rats with 2\u2009\u00d7\u20091 cm wounds were treated with the nanofibrous dressings, and wound healing progress was observed over a 14\u2010day period. qRT\u2010PCR was employed to analyze MMP\u20109, TIMP1, COL1A1, PDGFA, and VEGFC mRNA expressions. With its contemporary design surpassing existing treatments, the nanofiber wound dressing stands out for its wound\u2010healing acceleration and antibacterial properties.<\/jats:p>","DOI":"10.1002\/jbm.b.35544","type":"journal-article","created":{"date-parts":[[2025,1,31]],"date-time":"2025-01-31T13:13:46Z","timestamp":1738329226000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["A Cutting\u2010Edge Multilayer Nanofiber Wound Dressing: Design, Synthesis, and Investigation for Enhanced Wound Healing In\u00a0Vitro and In\u00a0Vivo"],"prefix":"10.1002","volume":"113","author":[{"given":"Irem Mukaddes","family":"Bilgiseven","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Science Faculty Selcuk University  Konya T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilyas","family":"Deveci","sequence":"additional","affiliation":[{"name":"Chemistry and Chemical Processing Technology Department, Technical Sciences Vocational School Konya Technical University  Konya T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kemal","family":"Kismet","sequence":"additional","affiliation":[{"name":"Department of Surgical Nursing, Faculty of Nursing Selcuk University  Konya T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4449-6103","authenticated-orcid":false,"given":"Serdar","family":"Karakurt","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Science Faculty Selcuk University  Konya T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,1,31]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2020.03.207"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsabm.8b00637"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/21691401.2019.1596933"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/mabi.201300561"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2020.02.022"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2013.12.015"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1590\/abd1806-4841.20164741"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2009.10.048"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1383\/surg.23.2.37.60352"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.b.34861"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xphs.2020.10.003"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xphs.2021.09.041"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.b.35435"},{"key":"e_1_2_11_15_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.msec.2020.110994","article-title":"Electrospinning of Natural Polymers for the Production of Nanofibres for Wound Healing Applications","volume":"114","author":"Juncos Bombin A. D.","year":"2020","journal-title":"Materials Science and Engineering C: Biomimetic and Supramolecular Systems"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1039\/c3tb20795a"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xphs.2023.12.013"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/app.54503"},{"key":"e_1_2_11_19_1","first-page":"1","article-title":"Trypan Blue Exclusion Test of Cell Viability","volume":"111","author":"Strober W.","year":"2001","journal-title":"Current Protocols in Immunology"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2023.e21560"},{"issue":"3","key":"e_1_2_11_21_1","first-page":"3831","article-title":"Antioxidant and Wound Healing Properties of \n                     Prosopis farcta\n                   and \n                     Adiantum capillus\n                   Plant Extracts: An in Vitro Study","volume":"14","author":"Mahmud S. O.","year":"2023","journal-title":"Journal of Pharmaceutical Negative Results"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.procbio.2023.04.029"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/wrr.13051"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2023.121307"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24119269"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-023-28065-6"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2021.01.209"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.15252\/msb.20178174"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.3c09394"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21249739"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2013.09.040"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2015.03.005"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.PRS.0000060102.57809.DA"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsabm.9b01147"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jss.2008.04.023"},{"issue":"3","key":"e_1_2_11_36_1","first-page":"187","article-title":"Quercetin Accelerated Cutaneous Wound Healing in Rats by Increasing Levels of VEGF and TGF\u2010\u03b21","volume":"54","author":"Gopalakrishnan A.","year":"2016","journal-title":"Indian Journal of Experimental Biology"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matlet.2024.136167"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.b.35370"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.4103\/jips.jips_364_22"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(03)00059-0"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2020.118062"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2023.113276"}],"container-title":["Journal of Biomedical Materials Research Part B: Applied Biomaterials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbm.b.35544","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,23]],"date-time":"2025-07-23T07:03:33Z","timestamp":1753254213000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbm.b.35544"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,31]]},"references-count":41,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2025,2]]}},"alternative-id":["10.1002\/jbm.b.35544"],"URL":"https:\/\/doi.org\/10.1002\/jbm.b.35544","archive":["Portico"],"relation":{},"ISSN":["1552-4973","1552-4981"],"issn-type":[{"value":"1552-4973","type":"print"},{"value":"1552-4981","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1,31]]},"assertion":[{"value":"2024-07-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-20","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-31","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e35544"}}