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Hemostatic nanofiber dressing using biogenic calcium powders from <jats:italic>Magallana cuttackensis<\/jats:italic> is reported here. Extracted and treated powders were characterized by XRD, EDX, XPS, and Raman spectroscopy to confirm the presence of CaO and Ca(OH)<jats:sub>2<\/jats:sub> as the calcium phases. A chitosan\u2010based nanofiber matrix, one of the most commonly used hemostatic dressing materials, was used as a control nanofiber (CNF) and integrated with shell waste calcium minerals (MSWNF). The hemolysis of CNF and MSWNF nanofibers was 5%\u2009\u00b1\u20090.3% and 4%\u2009\u00b1\u20090.2%, respectively. The in\u00a0vitro clotting time (CT) under CNF and MSWNF nanofibers was 230\u2009\u00b1\u200911\u2009s and 198\u2009\u00b1\u20094\u2009s, which, along with other parameters\u2014prothrombin times (27\u2009\u00b1\u20090.4\u2009s and 22\u2009\u00b1\u20090.6\u2009s) and plasma recalcification time (76\u2009\u00b1\u20094\u2009s and 47\u2009\u00b1\u20091\u2009s)\u2014indicated enhancement in hemostasis performance by MSWNF over CNF. After in\u00a0vivo evaluation in the rabbit incision model, the bleeding time of the control group (274\u2009\u00b1\u20096\u2009s) was longer than CNF (97\u2009\u00b1\u20098\u2009s) or MSWNF (75\u2009\u00b1\u20095\u2009s). Blood oozing until hemostasis was 0.380\u2009g, 0.354\u2009g, and 0.121\u2009g under control gauge, CNF, and MSWNF, respectively, demonstrating enhancement in hemostasis performance by MSWNF. Further, histopathological examination proved continued epithelialization and formation of capillaries, indicative of wound healing. The results provide promising evidence for a more effective biomaterial from biogenic oyster shell waste than commonly employed hemostatic dressing materials.<\/jats:p>","DOI":"10.1002\/jbm.b.35601","type":"journal-article","created":{"date-parts":[[2025,6,9]],"date-time":"2025-06-09T04:00:28Z","timestamp":1749441628000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Biogenic Calcium Mineral\u2010Chitosan Composite Nanofiber Dressing for Control of Traumatic Hemorrhage"],"prefix":"10.1002","volume":"113","author":[{"given":"Pranabesh Kumar","family":"Sasmal","sequence":"first","affiliation":[{"name":"Department of Mechanical Engineering Jadavpur University  Kolkata West Bengal India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shalini","family":"Dasgupta","sequence":"additional","affiliation":[{"name":"Centre for Healthcare Science and Technology Indian Institute of Engineering Science and 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