{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T15:26:06Z","timestamp":1791818766608,"version":"4.3.4"},"reference-count":36,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,7,31]],"date-time":"2025-07-31T00:00:00Z","timestamp":1753920000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biomed Mater Res"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Effective surface treatment of implants is essential for enhancing osseointegration outcomes. This study assessed the influence of alcohol decontamination both with and without secondary argon\u2010based non\u2010thermal plasma (NTP) treatment on osseointegration of endosteal implants in a large translational (sheep) model. Ti6Al4V dental implants were utilized either as received (CTRL), or subjected to ethanol cleaning (for 60\u2009s) followed by NTP (for 60\u2009s) (Clean+Plasma); or treated with NTP alone (Plasma) for 60\u2009s. X\u2010ray photoelectron spectroscopy was used for surface elemental analysis, followed by interferometry and sessile drop tests to measure changes in surface roughness and surface energy, respectively. Twelve sheep received implants (one implant per group per sheep) in the iliac crest, and bone healing was evaluated after 3 and 12\u2009weeks using histomorphometric analysis (six sheep\/time point). No significant differences in surface roughness (arithmetic mean (Sa) and root mean square (Sq) height: <jats:italic>p<\/jats:italic>\u2009&gt;\u20090.161 and <jats:italic>p<\/jats:italic>\u2009&gt;\u20090.173, respectively) or topographies were detected between implant surfaces. However, both NTP treated groups presented higher surface energies and lower water contact angle values relative to CTRL surface (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001). Compared to the CTRL, both NTP\u2010treated groups exhibited reduced levels of Carbon and elevated levels of Oxygen. No significant differences in Bone\u2010to\u2010Implant Contact (BIC) or Bone Area Fractional Occupancy (BAFO) were observed among groups at 3\u2009weeks. At the 12\u2010week time point, Plasma implants demonstrated significantly higher BAFO (<jats:italic>p<\/jats:italic>\u2009=\u20090.014) compared to the CTRL group, as well as an increase in both BIC and BAFO over time (3 vs. 12\u2009weeks in\u00a0vivo) (<jats:italic>p<\/jats:italic>\u2009=\u20090.041 and <jats:italic>p<\/jats:italic>\u2009=\u20090.043, respectively). Building on the existing literature, the current study suggests that NTP treatment alone may be adequate to successfully enhance osseointegration while minimizing contamination risks, thereby eliminating the need for additional cleaning protocols.<\/jats:p>","DOI":"10.1002\/jbm.b.35628","type":"journal-article","created":{"date-parts":[[2025,7,31]],"date-time":"2025-07-31T13:25:39Z","timestamp":1753968339000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Effect of Secondary Non\u2010Thermal Plasma Decontamination on Ethanol\u2010Treated Endosteal Implant Surfaces: An In\u00a0Vivo Study of Osseointegration"],"prefix":"10.1002","volume":"113","author":[{"given":"Adriana I.","family":"Sandino","sequence":"first","affiliation":[{"name":"University of Miami Miller School of Medicine  Miami Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joao Arthur","family":"Kawase De Queiroz Goncalves","sequence":"additional","affiliation":[{"name":"University of Miami Miller School of Medicine  Miami Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pawan","family":"Pathagamage","sequence":"additional","affiliation":[{"name":"University of Miami Miller School of Medicine  Miami Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baylee M.","family":"Brochu","sequence":"additional","affiliation":[{"name":"University of Miami Miller School of Medicine  Miami Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Savanah R.","family":"Sturm","sequence":"additional","affiliation":[{"name":"University of Miami Miller School of Medicine  Miami Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arthur","family":"Castellano","sequence":"additional","affiliation":[{"name":"Mackenzie Evangelical School of Medicine Paran\u00e1  Curitiba Brazil"},{"name":"Federal University of Paran\u00e1  Curitiba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2739-0339","authenticated-orcid":false,"given":"Vasudev Vivekanand","family":"Nayak","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology University of Miami Miller School of Medicine  Miami Florida USA"},{"name":"Dr. John T. Macdonald Foundation Biomedical Nanotechnology Institute (BioNIUM) University of Miami  Miami Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1458-6527","authenticated-orcid":false,"given":"Lukasz","family":"Witek","sequence":"additional","affiliation":[{"name":"Biomaterials and Regenerative Biology Division NYU College of Dentistry  New York New York USA"},{"name":"Department of Biomedical Engineering NYU Tandon School of Engineering  Brooklyn New York USA"},{"name":"Hansj\u00f6rg Wyss Department of Plastic Surgery NYU Grossman School of Medicine  New York New York USA"},{"name":"Department of Oral and Maxillofacial Surgery NYU College of Dentistry  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulo G.","family":"Coelho","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology University of Miami Miller School of Medicine  Miami Florida USA"},{"name":"Dr. John T. Macdonald Foundation Biomedical Nanotechnology Institute (BioNIUM) University of Miami  Miami Florida USA"},{"name":"DeWitt Daughtry Family Department of Surgery, Division of Plastic Surgery University of Miami Miller School of Medicine  Miami Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,31]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13191\u2010013\u20100252\u2010z"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmbbm.2007.12.002"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097\u20104636(199609)32:1<55::AID\u2010JBM7>3.0.CO;2\u2010O"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms241914688"},{"key":"e_1_2_7_6_1","doi-asserted-by":"crossref","unstructured":"M.Herrero\u2010Climent P.L\u00e1zaro J.Vicente Rios et\u00a0al. \u201cInfluence of Acid\u2010Etching After Grit\u2010Blasted on Osseointegration of Titanium Dental Implants: In\u00a0Vitro and In\u00a0Vivo Studies \u201d24(2013):2047\u20132055.","DOI":"10.1007\/s10856-013-4935-0"},{"issue":"6","key":"e_1_2_7_7_1","first-page":"779","article-title":"Surface Analysis of Machined Versus Sandblasted and Acid\u2010Etched Titanium Implants","volume":"15","author":"Orsini G.","year":"2000","journal-title":"International Journal of Oral and Maxillofacial Implants"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40729\u2010019\u20100204\u20104"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010023\u201041905\u20109"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dental.2016.11.011"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsbiomaterials.4c00081"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm9092817"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.11607\/ijp.7302"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21228598"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cpme.2016.01.001"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsbiomaterials.4c01413"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/app.1969.070130815"},{"issue":"6","key":"e_1_2_7_18_1","first-page":"1099","article-title":"Cell Behavior Related to Implant Surfaces With Different Microstructure and Chemical Composition: An In\u00a0Vitro Analysis","volume":"25","author":"Conserva E.","year":"2010","journal-title":"International Journal of Oral and Maxillofacial Implants"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010020\u201067678\u2010z"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/ma14081942"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.12403"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1088\/1367-2630\/11\/11\/115020"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1097\/bot.0000000000001908"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12447"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms12061137"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/bioengineering9030123"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/clr.12160"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2023.1137947"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.737635"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/femspd\/ftae007"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics11020200"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/JPER.18\u20100366"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010019\u201039414\u20109"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms242115889"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010020\u201059652\u20106"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/3873928"},{"key":"e_1_2_7_37_1","doi-asserted-by":"crossref","unstructured":"T.Du J.Ma P.Yang Z.Xiong X.Lu andY. 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