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These coatings can be easily modified to meet the requirements of specific cell types while preserving responsiveness. First, temperature\u2010responsive grafted copolymer brush coatings of poly(oligo(ethylene glycol) methyl ether methacrylate) (POEGMA) with a small amount of hydroxyethyl methacrylate (HEMA) were synthesized on glass surfaces. Subsequent modifications involved using multifunctional alcohols, amines, or their combinations with proteins to react with divinyl sulfone, forming a cross\u2010linked polymer matrix with a surface nanogel structure attached to grafted copolymer brushes containing hydroxyl groups. The resulting sandwich coatings were comprehensively characterized, revealing maintained temperature\u2010responsiveness for various structures of the grafted nanogel. Compared with P(OEGMA\u2010co\u2010HEMA) brushes, these temperature\u2010responsive sandwich coatings exhibited improved biocompatibility while retaining the ability to regulate cell morphology and detachment of dermal fibroblasts through external temperature control. Rheological analysis of live cells was performed on the developed platforms to reveal their impact on cellular behavior. The application of these new materials opens exciting possibilities for tissue engineering.<\/jats:p>","DOI":"10.1002\/jbm.b.35631","type":"journal-article","created":{"date-parts":[[2025,8,2]],"date-time":"2025-08-02T05:04:39Z","timestamp":1754111079000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["\u2018Smart\u2019 Polymer Sandwiches From Hydrogel Nanocoatings Attached to Stimuli\u2010Responsive Grafted Brush Coatings: Changing the Cell Behavior"],"prefix":"10.1002","volume":"113","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6342-6071","authenticated-orcid":false,"given":"Yana","family":"Shymborska","sequence":"first","affiliation":[{"name":"Jagiellonian University, Faculty of Physics, Astronomy and Applied Computer Science, Smoluchowski Institute of Physics  Krak\u00f3w Poland"},{"name":"Jagiellonian University, Doctoral School of Exact and Natural Sciences  Krak\u00f3w 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