{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:44:35Z","timestamp":1786977875337,"version":"build-2736575974"},"reference-count":31,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,8,6]],"date-time":"2025-08-06T00:00:00Z","timestamp":1754438400000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021856","name":"Ministero dell'Universit\u00e0 e della Ricerca","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100021856","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biomed Mater Res"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Managing bone defects is challenging, with autologous grafts being the most effective treatment. Biomaterials like alginate\/hydroxyapatite (Alg\/Hap) composites are increasingly used due to their biocompatibility and osteoconductive properties. Graft implantation in the oral cavity may trigger inflammatory responses, such as periodontitis, pulpitis, or caries, due to biomaterial recognition as non\u2010self and the presence of pathogens like\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Streptococcus mutans<\/jats:italic>\n                    <\/jats:styled-content>\n                    . Conjugating Alg\/Hap composites with antimicrobial silver nanoparticles (nAg) offers a strategy to counteract oral inflammation caused by microbial biofilms. This study explores the anti\u2010inflammatory and antibiofilm activities of these biomaterials during early implantation (24\u201372\u2009h), as well as DPSC viability and collagen expression. A co\u2010culture model of dental pulp stem cells (DPSCs) and\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Streptococcus mutans<\/jats:italic>\n                    <\/jats:styled-content>\n                    UA 159 strains was established.\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Streptococcus mutans<\/jats:italic>\n                    <\/jats:styled-content>\n                    viability and biofilm formation on scaffolds were evaluated through the live\/dead assay and confocal microscopy. Lactate dehydrogenase (LDH), interleukin\u20106 (IL\u20106), and collagen type 1 from DPSCs were measured via ELISA assays. Nrf2 and COX\u20102 protein expression was evaluated by western blotting. Alg\/Hap\/nAg composites reduce\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>S. mutans<\/jats:italic>\n                    <\/jats:styled-content>\n                    \u2010derived biofilm formation, preserving biocompatibility toward DPSCs. Decreased IL\u20106 levels, restored collagen type 1 secretion (5.98\u2009pg\/mL in DPSCs\u2010MOI 0.1\u2010Alg\/Hap\/Ag vs. 3.04\u2009pg\/mL in DPSCs\u2010Alg\/Hap\/Ag at 72\u2009h), and modulation of antioxidant and inflammatory proteins were observed, including a two\u2010fold increase of Nrf2 expression in cells seeded onto scaffolds in the presence of nAg. These findings highlight the potential of smart biomaterials to promote DPSC osteogenic and odontogenic differentiation, advancing oral tissue regeneration strategies.\n                  <\/jats:p>","DOI":"10.1002\/jbm.b.35636","type":"journal-article","created":{"date-parts":[[2025,8,6]],"date-time":"2025-08-06T09:57:48Z","timestamp":1754474268000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Cell Response Toward Inflammation of\n                    <scp>DPSCs<\/scp>\n                    Co\u2010Cultured With\n                    <scp>\n                      <i>Streptococcus mutans<\/i>\n                    <\/scp>\n                    in the Presence of Alginate\/Hydroxyapatite\u2010Based Scaffolds Enriched With Silver Nanoparticles"],"prefix":"10.1002","volume":"113","author":[{"given":"Valentina","family":"Puca","sequence":"first","affiliation":[{"name":"Department of Pharmacy University \u201cG. d'Annunzio\u201d of Chieti\u2010Pescara  Chieti Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noemi","family":"Mencarelli","sequence":"additional","affiliation":[{"name":"Department of Pharmacy University \u201cG. d'Annunzio\u201d of Chieti\u2010Pescara  Chieti Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benedetta","family":"Pellegrini","sequence":"additional","affiliation":[{"name":"Department of Pharmacy University \u201cG. d'Annunzio\u201d of Chieti\u2010Pescara  Chieti Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eleonora","family":"Marsich","sequence":"additional","affiliation":[{"name":"Department of Medicine, Surgery and Health Sciences University of Trieste  Trieste Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amelia","family":"Cataldi","sequence":"additional","affiliation":[{"name":"Department of Pharmacy University \u201cG. d'Annunzio\u201d of Chieti\u2010Pescara  Chieti Italy"},{"name":"UdA Tech Lab University \u201cG. d'Annunzio\u201d of Chieti\u2010Pescara  Chieti Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rossella","family":"Grande","sequence":"additional","affiliation":[{"name":"Department of Pharmacy University \u201cG. d'Annunzio\u201d of Chieti\u2010Pescara  Chieti Italy"},{"name":"Center for Advanced Studies and Technology (CAST) University \u201cG. d'Annunzio\u201d of Chieti\u2010Pescara  Chieti Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2283-4159","authenticated-orcid":false,"given":"Marialucia","family":"Gallorini","sequence":"additional","affiliation":[{"name":"Department of Pharmacy University \u201cG. d'Annunzio\u201d of Chieti\u2010Pescara  Chieti Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,8,6]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/039463200902200427"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0501.2011.02300.x"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20020435"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2019.01.061"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2018.07.017"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2021.118610"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/bm900154b"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/9643721"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2023.1257433"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40779-024-00532-9"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.4103\/0976\u2010237X.194114"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/app122211837"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00784-024-05687-2"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro.2016.94"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1080\/13543776.2020.1830060"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0096520"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/nano9121656"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10856-013-4923-4"},{"issue":"1","key":"e_1_2_10_20_1","article-title":"Streptococcus Mutans yidC1 and yidC2 Impact Cell Envelope Biogenesis, the Biofilm Matrix, and Biofilm Biophysical Properties","volume":"201","author":"Palmer S. 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