{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T04:35:21Z","timestamp":1792125321216,"version":"4.4.0"},"reference-count":38,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,10,18]],"date-time":"2025-10-18T00:00:00Z","timestamp":1760745600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biomed Mater Res"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Peripheral nerve injuries are a significant clinical concern, often resulting in incomplete functional recovery due to the limitations of current treatments. Biomaterial\u2010based scaffolds that mimic the mechanical and topographical features of native nerve tissue represent a promising strategy to support regeneration. In this study, we investigate the regenerative potential of soft and nerve\u2010mechanically compliant glycerol\u2010plasticized chitosan (Gly\u2013chi) microstructured membranes with in\u00a0vitro models with increasing biological complexity. These soft directional microgrooved membranes promoted invasion, polarization, and alignment of primary Schwann cells (SCs), and further promoted neurite outgrowth and directional guidance of human iPSC\u2010derived sensory neurons when co\u2010cultured on SCs. Moreover, tests with rat dorsal root ganglia (DRG) explants confirmed the ability of these scaffolds to orient axonal extension also in an ex\u00a0vivo setting. Interestingly, neurites aligned even over a confluent SC layer, indicating that topographical cues may be transmitted via SC\u2010mediated signaling in addition to direct contact. Overall, our findings demonstrate that glycerol\u2010blended chitosan membranes with a physiological\u2010grade stiffness, similar in respect to nerve tissues, and micro\u2010structured with directional grooves effectively support both glial and neuronal organization and represent a robust biomimetic platform for peripheral nerve repair and advanced in\u00a0vitro modeling applications.<\/jats:p>","DOI":"10.1002\/jbm.b.35660","type":"journal-article","created":{"date-parts":[[2025,10,18]],"date-time":"2025-10-18T14:00:42Z","timestamp":1760796042000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Soft Scaffolds for Nerve Repair: Investigating Glycerol\u2010Plasticized Chitosan Microstructures With In\u00a0Vitro Complex 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