{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,3]],"date-time":"2025-12-03T07:41:48Z","timestamp":1764747708054,"version":"3.46.0"},"reference-count":43,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T00:00:00Z","timestamp":1760918400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005378","name":"Hacettepe \u00dcniversitesi","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005378","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biomed Mater Res"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This study aimed to biologically modify polyetheretherketone (PEEK) using cell sheets after chemical modification, with the goal of enhancing its bioactivity and improving its integration with dental tissue. To achieve this, sulfonated PEEK (SPEEK) and\/or boron\u2010doped nanohydroxyapatite (B\u2010nHAp)\u2010coated SPEEK (SPEEK\u2010B\u2010nHAp) materials were combined with periodontal ligament (PDL) or PDL\u2010co\u2010human umbilical vein endothelial (HUVEC) cell sheets. PDL and PDL\u2010co\u2010HUVEC cell sheets were obtained from 12\u2010well plates over a 4\u2010day period using L\u2010ascorbic acid induction (100\u2009\u03bcg\/mL) and a concentrated cell density of 1.0\u2009\u00d7\u200910\n                    <jats:sup>5<\/jats:sup>\n                    cells\/cm\n                    <jats:sup>2<\/jats:sup>\n                    . Both PDL and co\u2010culture cell sheets adhered well to and proliferated on the surfaces of SPEEK and SPEEK\u2010B\u2010nHAp. However, the co\u2010culture cell sheet groups showed faster growth compared to the PDL cell sheet groups on both SPEEK and SPEEK\u2010B\u2010nHAp materials at each time point. Western blot and RT\u2010PCR analyses revealed a significant increase in early\u2010 and mid\u2010term osteogenic markers in PDL cell sheets on both SPEEK and SPEEK\u2010B\u2010nHAp surfaces. Additionally, late\u2010stage osteogenic and angiogenic markers were enhanced in the co\u2010culture cell sheets on both SPEEK and SPEEK\u2010B\u2010nHAp samples. This study concluded that PDL\u2010co\u2010HUVEC cell sheets covering SPEEK\u2010B\u2010nHAp surfaces can effectively support bone\u2010implant integration and represent a promising modification approach for PEEK dental implants.\n                  <\/jats:p>","DOI":"10.1002\/jbm.b.35665","type":"journal-article","created":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T03:36:34Z","timestamp":1761017794000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Development of Ligament\u2010Anchored Dental Implants Using Cell Sheets on Sulfonated\n                    <scp>PEEK<\/scp>\n                    Surfaces Coated With Boron\u2010Doped Nano\u2010Hydroxyapatite"],"prefix":"10.1002","volume":"113","author":[{"given":"\u00dclk\u00fc","family":"\u00c7ay\u0131r","sequence":"first","affiliation":[{"name":"Graduate School of Science and Engineering, Bioengineering Division Hacettepe University  Ankara 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