{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T15:13:44Z","timestamp":1761664424969,"version":"build-2065373602"},"reference-count":54,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,10,5]],"date-time":"2025-10-05T00:00:00Z","timestamp":1759622400000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000090","name":"Congressionally Directed Medical Research Programs","doi-asserted-by":"publisher","award":["W81XWH\u201020\u20101\u20100378"],"award-info":[{"award-number":["W81XWH\u201020\u20101\u20100378"]}],"id":[{"id":"10.13039\/100000090","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biomed Mater Res"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Traumatic extremity injuries suffer a high probability of infection and often amputation due to contamination and delays in treatment. Military service members are predisposed to injury while engaged in conflict, yet current military adherence to antibiotic administration protocols following traumatic injury is lacking. Moreover, systemic antibiotic prophylaxis might not effectively eradicate biofilm throughout the wound site. Previously, an antibiotic wound gel was created to address current limitations of prophylactic antibiotic treatment in austere environments, particularly the battlefield, by offering a simple solution to control the release of tobramycin over a one\u2010week period. We hypothesized that tobramycin eluted from the gel would effectively manage biofilm\u2010related infection when tested in a large animal model of traumatic long\u2010bone injury. Sheep were either treated with tobramycin\u2010loaded gel or gel alone, and the reduction in bioburden was determined by quantifying tissue and inoculation substrates after a one\u2010week period. Results indicated the wound gel was effective at managing biofilm in this model, with no detectable growth observed in tissues collected from treated animals. Further, the antibiotic\u2010loaded wound gel significantly reduced the severity of the inflammatory response in the surrounding tissue. Biofilm presence was confirmed in scanning electron and light microscopy images of tissues treated with gel alone. Additionally, reactive bone growth, a characteristic of biofilm infection, was consistently observed in all untreated animals but appeared effectively managed in those treated with the antibiotic wound gel. Localized delivery of a broad\u2010spectrum antibiotic from a controlled\u2010release gel can improve adherence to antibiotic administration guidelines and has a greater potential to stabilize biofilm\u2010contaminated wound sites quickly after injury while also mitigating a severe inflammatory response.<\/jats:p>","DOI":"10.1002\/jbm.b.35669","type":"journal-article","created":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T06:41:37Z","timestamp":1759732897000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["In Vivo Efficacy of an Antibiotic Wound Gel in a Sheep Model of Bone Trauma and Biofilm\u2010Related Infection"],"prefix":"10.1002","volume":"113","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7768-8972","authenticated-orcid":false,"given":"Annika L.","family":"Gilmore","sequence":"first","affiliation":[{"name":"Department of Orthopaedics University of Utah  Salt Lake City Utah USA"},{"name":"Bone and 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Salt Lake City Utah USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicholas N.","family":"Ashton","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics University of Utah  Salt Lake City Utah USA"},{"name":"Bone and Biofilm Research Lab University of Utah  Salt Lake City Utah USA"},{"name":"Department of Biomedical Engineering University of Utah  Salt Lake City Utah USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric","family":"Cozzone","sequence":"additional","affiliation":[{"name":"DePuy Synthes  West Chester Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charles A.","family":"Florek","sequence":"additional","affiliation":[{"name":"DePuy Synthes  West Chester Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-2854-8832","authenticated-orcid":false,"given":"David 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