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To optimize dressing thickness for partial\u2010thickness skin wounds and evaluate translational relevance. We compared PCL dressings with areal weights of 10, 20, and 30\u2009g\/m\n                    <jats:sup>2<\/jats:sup>\n                    (with or without 7\u2009wt.% LPPO) in a porcine model using partial\u2010thickness wounds. Standard comparators were Aquacel Ag\n                    <jats:sup>+<\/jats:sup>\n                    (antibacterial control) and Jelonet (standard control). Healing was assessed macroscopically and histologically (hematoxylin and eosin, keratins\u201010 and \u201014 immunohistochemistry). Systemic LPPO exposure was measured by LC\u2013MS\/MS in plasma and liver. A clinical case (STSG donor site) used a 15\u2009g\/m\n                    <jats:sup>2<\/jats:sup>\n                    NANO dressing. Thinner dressings (10 and 20\u2009g\/m\n                    <jats:sup>2<\/jats:sup>\n                    ), whether unloaded (NANO) or LPPO\u2010loaded (NANO\u2010LPPO), supported rapid re\u2010epithelialization from wound edges and hair follicles and yielded complete closure in wounds, comparable to controls. The 30\u2009g\/m\n                    <jats:sup>2<\/jats:sup>\n                    variants of both NANO and NANO\u2010LPPO were associated with persistent inflammation and delayed re\u2010epithelialization. LC\u2013MS\/MS showed plasma concentrations of LPPO below the quantification limit and very low liver levels. The clinical case mirrored the porcine findings: timely re\u2010epithelialization of the STSG donor site with 15\u2009g\/m\n                    <jats:sup>2<\/jats:sup>\n                    NANO was comparable to Jelonet. Dressing thickness influenced healing quality in the porcine model. Partial\u2010thickness wounds treated with 10\u201320\u2009g\/m\n                    <jats:sup>2<\/jats:sup>\n                    NANO dressings showed superior outcomes compared with 30\u2009g\/m\n                    <jats:sup>2<\/jats:sup>\n                    , with negligible systemic LPPO exposure. In the clinical case, a 15\u2009g\/m\n                    <jats:sup>2<\/jats:sup>\n                    NANO dressing supported timely re\u2010epithelialization of the STSG donor site, comparable to standard care. These findings justify further clinical development of PCL NANO\u2010LPPO dressings.\n                  <\/jats:p>","DOI":"10.1002\/jbm.b.70054","type":"journal-article","created":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T06:48:20Z","timestamp":1773384500000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Thickness\u2010Dependent Healing Effects of Polycaprolactone Electrospun Nanofiber Dressings (Loaded With Lipophosphonoxin) in Partial\u2010Thickness Skin Wounds"],"prefix":"10.1002","volume":"114","author":[{"given":"Hubert","family":"\u0160uca","sequence":"first","affiliation":[{"name":"Prague Burn Centre, Third Faculty of Medicine and University Hospital Kr\u00e1lovsk\u00e9 Vinohrady  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jana","family":"Sabov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Faculty of Medicine Pavol Jozef \u0160af\u00e1rik University  Ko\u0161ice Slovak Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u0160tefan","family":"Juh\u00e1s","sequence":"additional","affiliation":[{"name":"Institute of Animal Physiology and Genetics, Czech Academy of Sciences, v.v.i  Lib\u011bchov Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jana","family":"Juh\u00e1sov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Animal Physiology and Genetics, Czech Academy of Sciences, v.v.i  Lib\u011bchov Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V\u011bra","family":"Jen\u010dov\u00e1","sequence":"additional","affiliation":[{"name":"Faculty of Science Humanities and Education, Technical University of Liberec  Liberec Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva Ku\u017eelov\u00e1","family":"Ko\u0161\u0165\u00e1kov\u00e1","sequence":"additional","affiliation":[{"name":"Faculty of Science Humanities and Education, Technical University of Liberec  Liberec Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nitjawan","family":"Sahatsapan","sequence":"additional","affiliation":[{"name":"Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucia","family":"Fr\u00f6hlichov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Pathology Louis Pasteur University Hospital and Pavol Jozef \u0160af\u00e1rik University  Ko\u0161ice Slovak Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maxim","family":"Lisenko","sequence":"additional","affiliation":[{"name":"Faculty of Science Humanities and Education, Technical University of Liberec  Liberec Czech 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