{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T12:46:10Z","timestamp":1782477970965,"version":"3.54.5"},"reference-count":33,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T00:00:00Z","timestamp":1780444800000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biomed Mater Res"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Targeted drug delivery remains a major challenge in the treatment of colorectal cancer (CRC), particularly in overcoming tumor immune evasion. Exosome\u2010based nanocarriers have shown considerable promise due to their tumor\u2010targeting capability and immunomodulatory potential. In this study, EGFR\u2010targeted RNA\u2013exosome composite paclitaxel (PTX) nanoparticles (RNA\u2010Exo\u2010PTX) were constructed and characterized. Their antitumor effects and mechanisms were evaluated using colorectal cancer cell models, T\u2010cell co\u2010culture systems, and nude mouse xenografts. In\u00a0vitro assays, flow cytometry, ELISA, and molecular analyses were used to assess cell proliferation, apoptosis, migration, and immune\u2010related responses, while in\u00a0vivo effects were examined by RT\u2010qPCR, Western blotting, immunofluorescence, and immunohistochemistry. RNA\u2010Exo\u2010PTX exhibited a uniform size distribution, preserved exosomal characteristics, and demonstrated high RNA loading efficiency. Compared with free paclitaxel, RNA\u2010Exo\u2010PTX markedly inhibited colorectal cancer cell proliferation, migration, and clonogenic capacity while promoting apoptosis; these effects were partially attenuated upon EGFR or PRELP interference. In the T\u2010cell co\u2010culture system, RNA\u2010Exo\u2010PTX treatment significantly upregulated PRELP expression in colorectal cancer cells and enhanced T\u2010cell activation, as evidenced by increased secretion of IFN\u2010\u03b3, granzyme B, and perforin, along with elevated CD4\n                    <jats:sup>+<\/jats:sup>\n                    and CD8\n                    <jats:sup>+<\/jats:sup>\n                    T\u2010cell signals. In\u00a0vivo experiments showed that EGFR\u2010targeted RNA\u2010Exo\u2010PTX effectively suppressed tumor progression and reduced PD\u2010L1 and Ki\u201067 expression in tumor tissues, accompanied by upregulation of PTEN and inhibition of AKT\/mTOR signaling. Blockade of EGFR weakened the antitumor effects, whereas PRELP overexpression further enhanced therapeutic efficacy. EGFR\u2010targeted RNA\u2013exosome composite paclitaxel nanoparticles effectively suppress colorectal cancer progression and attenuate immune evasion, highlighting their potential value in targeted chemotherapy and immunomodulatory strategies for CRC.\n                  <\/jats:p>","DOI":"10.1002\/jbm.b.70111","type":"journal-article","created":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T07:25:47Z","timestamp":1780471547000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>EGFR<\/scp>\n                    \u2010Targeted\n                    <scp>RNA<\/scp>\n                    \u2013Exosome Nanoparticles Delivering Paclitaxel Suppress Colorectal Cancer via\n                    <scp>PRELP<\/scp>\n                    Upregulation"],"prefix":"10.1002","volume":"114","author":[{"given":"QingHan","family":"Li","sequence":"first","affiliation":[{"name":"Department of Hepatobiliary and Pancreatic Surgery First People's Hospital of Foshan  Foshan Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-0050-460X","authenticated-orcid":false,"given":"Yong","family":"Ji","sequence":"additional","affiliation":[{"name":"Department of Gastrointestinal Surgery First People's Hospital of Foshan  Foshan Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ZuoJun","family":"Zhen","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary and Pancreatic Surgery First People's Hospital of Foshan  Foshan Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"YinTao","family":"He","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary and Pancreatic Surgery First People's Hospital of Foshan  Foshan Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,3]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21772"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21660"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.abl3649"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13046\u2010024\u201003085\u2010w"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.25163\/angiotherapy.88109973"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2025.1684006"},{"issue":"7","key":"e_1_2_13_8_1","first-page":"3445","article-title":"Nab\u2010Paclitaxel Promotes the Cancer\u2010Immunity Cycle as a Potential Immunomodulator","volume":"11","author":"Chen Y.","year":"2021","journal-title":"American Journal of Cancer Research"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12951\u2010025\u201003352\u20108"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12929\u2010024\u201001055\u20100"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25073645"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.650426"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2024.1447953"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.1039\/c9lc00958b"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nano.2023.102667"},{"key":"e_1_2_13_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms222312828"},{"key":"e_1_2_13_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13046\u2010025\u201003277\u2010y"},{"key":"e_1_2_13_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2019.12.006"},{"key":"e_1_2_13_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2024.10.015"},{"key":"e_1_2_13_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010024\u201080879\u20100"},{"key":"e_1_2_13_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12953\u2010022\u201000190\u20106"},{"key":"e_1_2_13_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12967\u2010023\u201004476\u2010x"},{"key":"e_1_2_13_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2025.1615259"},{"key":"e_1_2_13_24_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.204980"},{"key":"e_1_2_13_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10495\u2010024\u201002015\u20107"},{"key":"e_1_2_13_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010025\u201058992\u2010z"},{"key":"e_1_2_13_27_1","doi-asserted-by":"publisher","DOI":"10.1039\/d4na00501e"},{"key":"e_1_2_13_28_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells12101416"},{"key":"e_1_2_13_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/s44222\u2010023\u201000138\u20101"},{"key":"e_1_2_13_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdl138"},{"key":"e_1_2_13_31_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M117.811299"},{"key":"e_1_2_13_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2022.110950"},{"key":"e_1_2_13_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2019.09.030"},{"key":"e_1_2_13_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers17233803"}],"container-title":["Journal of Biomedical Materials Research Part B: Applied Biomaterials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbm.b.70111","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jbm.b.70111","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbm.b.70111","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T11:52:27Z","timestamp":1782474747000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbm.b.70111"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6]]},"references-count":33,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["10.1002\/jbm.b.70111"],"URL":"https:\/\/doi.org\/10.1002\/jbm.b.70111","archive":["Portico"],"relation":{},"ISSN":["1552-4973","1552-4981"],"issn-type":[{"value":"1552-4973","type":"print"},{"value":"1552-4981","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6]]},"assertion":[{"value":"2026-04-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-27","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70111"}}