{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T14:27:01Z","timestamp":1786112821714,"version":"build-2736575974"},"reference-count":60,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T00:00:00Z","timestamp":1784592000000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000199","name":"U.S. Department of Agriculture","doi-asserted-by":"publisher","award":["2023\u201067013\u201039909"],"award-info":[{"award-number":["2023\u201067013\u201039909"]}],"id":[{"id":"10.13039\/100000199","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["2414448"],"award-info":[{"award-number":["2414448"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biomed Mater Res"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Osteomyelitis is a significant clinical challenge that leads to bone destruction and loss. Systemic antibiotic treatments do not reach infected sites at therapeutic levels, necessitating high dosages, which in turn can lead to toxicity and promote the emergence of antibiotic\u2010resistant bacteria. To overcome these challenges, our objective was to synthesize a biocompatible drug\u2010delivery system that has the potential to achieve localized antibiotic delivery to completely eradicate bacteria. We synthesized four formulations of microparticles using thiol\u2010ene click chemistry by varying the thiol monomer\u2010to\u2010crosslinker (2\u2010thiol:4\u2010thiol) ratio and the ene monomer\u2010to\u2010surfactant (2\u2010ene:Hitenol) ratio. The microparticles were loaded with doxycycline by soaking them in 20 or 50\u2009mg\/mL doxycycline (D20 or D50) over 24\u2009h. The microparticles exhibited a payload\u2010dependent release, with the D20 groups releasing approximately 11%\u201322% of their total doxycycline content, while the D50 groups released less than 10% of the total doxycycline content by 96\u2009h. All doxycycline\u2010loaded microparticle formulations showed antibacterial activity against Methicillin\u2010resistant\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    <\/jats:styled-content>\n                    (MRSA) over a 7\u2010day period, with the formulation with a 2\u2010thiol:4\u2010thiol ratio of 0.1:0.9 and a 2\u2010ene:Hitenol ratio of 0.8:0.2 showing complete eradication of bacteria by Day 7. The MTT and Live\/Dead assays showed that doses of 12\u2009mg or less of this formulation were not cytotoxic to mouse pre\u2010osteoblast cells. Taken together, we successfully synthesized a biocompatible microparticle\u2010based drug delivery system that can be loaded with doxycycline and that exhibits drug release under physiological conditions.\n                  <\/jats:p>","DOI":"10.1002\/jbm.b.70132","type":"journal-article","created":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T06:45:36Z","timestamp":1784702736000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Synthesis and Characterization of Thiol\u2010Ene Microparticles for Doxycycline Delivery"],"prefix":"10.1002","volume":"114","author":[{"given":"Chipo","family":"Chapusha","sequence":"first","affiliation":[{"name":"Department of Biomedical Materials Science, School of Dentistry University of Mississippi Medical Center  Jackson Mississippi USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas","family":"McGowan","sequence":"additional","affiliation":[{"name":"Department of Biomedical Materials Science, School of Dentistry University of Mississippi Medical Center  Jackson Mississippi USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paige","family":"Smith","sequence":"additional","affiliation":[{"name":"Department of Biomedical Materials Science, School of Dentistry University of Mississippi Medical Center  Jackson Mississippi USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary","family":"Carr","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Biology, School of Medicine University of Mississippi Medical Center  Jackson Mississippi USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary E.","family":"Marquart","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Biology, School of Medicine University of Mississippi Medical Center  Jackson Mississippi USA"},{"name":"Center for Immunology and Microbial Research (CIMR) University of Mississippi Medical Center  Jackson Mississippi USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jared","family":"Cobb","sequence":"additional","affiliation":[{"name":"Environmental Laboratory Engineer Research and Development Center, U.S. Army Corps of Engineers  Vicksburg Mississippi USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3937-2149","authenticated-orcid":false,"given":"Amol V.","family":"Janorkar","sequence":"additional","affiliation":[{"name":"Department of Biomedical Materials Science, School of Dentistry University of Mississippi Medical Center  Jackson Mississippi USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,21]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11914\u2010019\u201000548\u20104"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00084-17"},{"key":"e_1_2_10_4_1","volume-title":"Osteomyelitis Ifeanyi I. 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