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A BMP\u20102\u2010loaded mPEG\u2010PLGA\u2010BOX thermosensitive hydrogel was developed and characterized in\u00a0vitro for its rheological properties, degradation behavior, and drug\u2010release kinetics. The feasibility of ultrasound\u2010triggered release was assessed using various duty cycles. Subsequently, therapeutic efficacy was evaluated in a monoiodoacetate\u2010induced rat model of osteoarthritis. Treatment groups included untreated control (MIA), free BMP\u20102 (MB), passive BMP\u20102\u2010hydrogel (MHB), and BMP\u20102\u2010loaded hydrogel with ultrasound stimulation (MHBU). Outcomes were assessed up to day 49 using functional gait analysis (CatWalk), histopathology (H&amp;E, Toluidine Blue), and immunohistochemistry for Collagen II, Collagen X, and Sox 9. The in\u00a0vitro analysis supported the selection of a non\u2010thermal 5% ultrasound duty cycle, which produced an approximately 550\u2010fold ultrasound\u2010enhanced release ratio without a detectable temperature increase while limiting residual\u2010volume loss relative to the higher\u2010duty\u2010cycle conditions. In\u00a0vivo, the MHBU therapy was uniquely effective. It was the only intervention to significantly restore articular cartilage thickness (212.8\u2009\u00b1\u200942.0\u2009\u03bcm vs. 124.3\u2009\u00b1\u200912.7\u2009\u03bcm,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.01), achieving a level statistically indistinguishable from healthy controls. This structural repair was directly correlated with normalized dynamic gait parameters (print area, stance) and a restored tissue\u2010level profile, characterized by enhanced Collagen II matrix, suppressed pathological Collagen X expression, and recovered chondrogenic Sox 9 expression. This study demonstrates that an ultrasound\u2010responsive hydrogel platform successfully overcomes the limitations of both conventional growth factor delivery (rapid clearance) and passive hydrogel encapsulation (slow release). This synergistic, ultrasound\u2010triggered system promotes significant functional and structural cartilage restoration, representing a promising translatable strategy for osteoarthritis therapy.\n                  <\/jats:p>","DOI":"10.1002\/jbm.b.70158","type":"journal-article","created":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T04:13:48Z","timestamp":1790741628000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Therapeutic Potential of Ultrasound\u2010Controlled\n                    <scp>BMP<\/scp>\n                    \u20102 Release From Thermoresponsive Hydrogels in a Rat Knee Osteoarthritis Model"],"prefix":"10.1002","volume":"114","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8772-1200","authenticated-orcid":false,"given":"Yi","family":"Kung","sequence":"first","affiliation":[{"name":"Department of Biomechatronic Engineering National Chiayi University  Chiayi city 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