{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T02:54:17Z","timestamp":1790909657674,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biomed Mater Res"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Decellularized extracellular matrix\u2010based scaffolds have gained significant attention for tissue regeneration applications. However, there is a limited understanding of how storage conditions influence the rheological and biological properties of decellularized matrix\u2010based injectable formulations. This study investigates the effects of different storage conditions (4\u00b0C, \u221280\u00b0C, and freeze\u2010dried) on the rheological properties of a decellularized porcine peripheral nerve\u2010derived extracellular matrix hydrogel. Our results demonstrated that, compared to storage at 4\u00b0C, formulations stored at \u221280\u00b0C and freeze\u2010dried maintained comparable gelation kinetics (complete gelation within 11\u2009min), mechanical properties (storage modulus around 300\u2013350 Pa), and shear\u2010thinning behavior across multiple time\u2010points (Days 0, 3, 7, and 14 for \u221280\u00b0C), similar to freshly prepared formulations at Day 0. Solution stored at 4\u00b0C demonstrated a decrease in mechanical stiffness of hydrogel with time, however their gelation kinetics remained similar to Day 0. The solutions stored at \u221280\u00b0C and resuspended freeze\u2010dried solutions maintained their complex viscosity, while 4\u00b0C stored solutions had relatively low complex viscosity as compared to freshly digested solutions. Overall, our findings suggest that \u221280\u00b0C storage and freeze\u2010drying are suitable approaches for preserving decellularized porcine peripheral nerve\u2010derived extracellular matrix formulations. Given the translational potential of these injectable biomaterials, understanding the influence of storage conditions on rheological performance is critical for developing appropriate storage and shipping strategies.<\/jats:p>","DOI":"10.1002\/jbm.b.70161","type":"journal-article","created":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T02:32:52Z","timestamp":1790908372000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effect of Storage Conditions on Rheological Properties of Decellularized Extracellular Matrix\u2010Based Injectable Hydrogels"],"prefix":"10.1002","volume":"114","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7906-9611","authenticated-orcid":false,"given":"Gopal","family":"Agarwal","sequence":"first","affiliation":[{"name":"J. Crayton Pruitt Family Department of Biomedical Engineering, Herbert Wertheim College of Engineering University of Florida  Gainesville Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leina","family":"Owens","sequence":"additional","affiliation":[{"name":"J. Crayton Pruitt Family Department of Biomedical Engineering, Herbert Wertheim College of Engineering University of Florida  Gainesville Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-8906-6762","authenticated-orcid":false,"given":"Khadeejah","family":"Ahmed","sequence":"additional","affiliation":[{"name":"J. Crayton Pruitt Family Department of Biomedical Engineering, Herbert Wertheim College of Engineering University of Florida  Gainesville Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5865-6016","authenticated-orcid":false,"given":"Christine E.","family":"Schmidt","sequence":"additional","affiliation":[{"name":"J. Crayton Pruitt Family Department of Biomedical Engineering, Herbert Wertheim College of Engineering University of Florida  Gainesville Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,1]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mtbio.2023.100589"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.reth.2021.04.002"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.36688"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2023.114506"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1088\/1741\u20102552\/ad5939"},{"key":"e_1_2_8_7_1","doi-asserted-by":"crossref","unstructured":"G.Agarwal A. 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