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Here, we describe the short\u2010term effects of different bisphosphonates on controlling the bone resorptive activity of mature osteoclasts in living bone tissues of mice using intravital two\u2010photon microscopy with a pH\u2010sensing chemical fluorescent probe. Three types of nitrogen\u2010containing bisphosphonates, risedronate, alendronate, and minodronate, inhibited osteoclastic acidification during osteoporotic conditions just 12 hours after i.v. injection. Among the three types of drugs, risedronate was the most effective at increasing osteoclast motility and changing the localization of proton pumps, which led to an inhibition of bone resorption. Together, these results demonstrate that the intravital imaging system is a useful tool for evaluating the similarities and differences in currently used antibone resorptive drugs. \u00a9 2018 The Authors. <jats:italic>JBMR Plus<\/jats:italic> published by Wiley Periodicals, Inc. on behalf of American Society for Bone and Mineral Research.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jbm4.10057","type":"journal-article","created":{"date-parts":[[2018,5,7]],"date-time":"2018-05-07T17:49:57Z","timestamp":1525715397000},"page":"362-366","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Dynamic Analyses of the Short\u2010Term Effects of Different Bisphosphonates Using Intravital Two\u2010Photon Microscopy"],"prefix":"10.1093","volume":"2","author":[{"given":"Junichi","family":"Kikuta","sequence":"first","affiliation":[{"name":"Department of Immunology and Cell Biology Graduate School of Medicine and Frontier Biosciences Osaka University 2\u20102 Yamada\u2010oka Suita Osaka 565\u20100871 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mai","family":"Shirazaki","sequence":"additional","affiliation":[{"name":"Department of Immunology and Cell Biology Graduate School of Medicine and Frontier Biosciences Osaka University 2\u20102 Yamada\u2010oka Suita Osaka 565\u20100871 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takao","family":"Sudo","sequence":"additional","affiliation":[{"name":"Department of Immunology and Cell Biology Graduate School of Medicine and Frontier Biosciences Osaka University 2\u20102 Yamada\u2010oka Suita Osaka 565\u20100871 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroki","family":"Mizuno","sequence":"additional","affiliation":[{"name":"Department of Immunology and Cell Biology Graduate School of Medicine and Frontier Biosciences Osaka University 2\u20102 Yamada\u2010oka Suita Osaka 565\u20100871 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