{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T18:02:13Z","timestamp":1791223333746,"version":"4.1.0"},"reference-count":57,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2020,3,25]],"date-time":"2020-03-25T00:00:00Z","timestamp":1585094400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["K23\u2010DK080139"],"award-info":[{"award-number":["K23\u2010DK080139"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["asbmr.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["JBMR Plus"],"published-print":{"date-parts":[[2020,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>A main obstacle to diagnose and manage renal osteodystrophy (ROD) is the identification of intracortical bone turnover type (low, normal, high). The gold standard, tetracycline\u2010labeled transiliac crest bone biopsy, is impractical to obtain in most patients. The Kidney Disease Improving Global Outcomes Guidelines recommend PTH and bone\u2010specific alkaline phosphatase (BSAP) for the diagnosis of turnover type. However, PTH and BSAP have insufficient diagnostic accuracy to differentiate low from non\u2010low turnover and were validated for trabecular turnover. We hypothesized that four circulating microRNAs (miRNAs) that regulate osteoblast (miRNA\u201030b, 30c, 125b) and osteoclast development (miRNA\u2010155) would provide superior discrimination of low from non\u2010low turnover than biomarkers in clinical use. In 23 patients with CKD 3\u20105D, we obtained tetracycline\u2010labeled transiliac crest bone biopsy and measured circulating levels of intact PTH, BSAP, and miRNA\u201030b, 30c, 125b, and 155. Spearman correlations assessed relationships between miRNAs and histomorphometry and PTH and BSAP. Diagnostic test characteristics for discriminating low from non\u2010low intracortical turnover were determined by receiver operator curve analysis; areas under the curve (AUC) were compared by \u03c7<jats:sup>2<\/jats:sup>test. In CKD rat models of low and high turnover ROD, we performed histomorphometry and determined the expression of bone tissue miRNAs. Circulating miRNAs moderately correlated with bone formation rate and adjusted apposition rate at the endo\u2010 and intracortical envelopes (\u03c1 = 0.43 to 0.51;<jats:italic>p<\/jats:italic>&lt;\u20090.05). Discrimination of low versus non\u2010low turnover was 0.866, 0.813, 0.813, and 0.723 for miRNA\u201030b, 30c, 125b, and 155, respectively, and 0.509 and 0.589 for PTH and BSAP, respectively. For all four miRNAs combined, the AUC was 0.929, which was superior to that of PTH and BSAP alone and together (<jats:italic>p<\/jats:italic>&lt;\u20090.05). In CKD rats, bone tissue levels of the four miRNAs reflected the findings in human serum. These data suggest that a panel of circulating miRNAs provide accurate noninvasive identification of bone turnover in ROD. \u00a9 2020 The Authors.<jats:italic>JBMR Plus<\/jats:italic>published by Wiley Periodicals, Inc. on behalf of American Society for Bone and Mineral Research.<\/jats:p>","DOI":"10.1002\/jbm4.10353","type":"journal-article","created":{"date-parts":[[2020,2,25]],"date-time":"2020-02-25T19:56:56Z","timestamp":1582660616000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["A microRNA Approach to Discriminate Cortical Low Bone Turnover in Renal Osteodystrophy"],"prefix":"10.1093","volume":"4","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8870-1896","authenticated-orcid":false,"given":"Thomas L","family":"Nickolas","sequence":"first","affiliation":[{"name":"Department of Medicine Columbia University Medical Center New York NY 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Leuven Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick C","family":"D'Haese","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Laboratory of Pathophysiology Antwerp University Wilrijk Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabrice","family":"Mac\u2010Way","sequence":"additional","affiliation":[{"name":"CHU de Qu\u00e9bec Research Center, L'H\u00f4tel\u2010Dieu de Qu\u00e9bec Hospital, Endocrinology and Nephrology Axis, Faculty and Department of Medicine Universit\u00e9 Laval Quebec City Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7902-2127","authenticated-orcid":false,"given":"Rosa","family":"Moyses","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Investiga\u00e7\u00e3o M\u00e9dica 16 Hospital das Clinicas da Faculdade de Medicina da Universidade de Sao Paulo Sao Paulo 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